dried food! What are the types and characteristics of biological buffers?

Due to the fact that most experimental types of cells can only operate within a narrow pH range, a certain buffer system is needed to maintain the changes that occur during the metabolic process, and the buffer solution has the effect of stabilizing pH changes, which has become a consensus choice among the public. However, due to the wide variety and use range of buffer solutions, it is usually necessary to choose according to experimental requirements. Below, we will focus on introducing several common types of buffer solutions, which can also be quickly identified during later use.

1、 PBS (phosphate) buffer

Due to secondary dissociation, the pH value of PBS is relatively broad, and it can be used to prepare acidic, alkaline, and neutral solutions with different pH values. To prepare an acidic solution, sodium dihydrogen phosphate can be directly added with a pH value of around 1-5. If an alkaline solution is prepared, potassium dihydrogen phosphate can be directly used with a pH range of 9-12.

Advantages: It is relatively easy to prepare solutions of different concentrations, and has a wide range of pH values, which are less affected by temperature.

2、 Tris buffer

As a weakly alkaline solution, it is generally used within the "neutral" range, such as preparing Tris HCl solution with a pH value of around 7.5 and a triphosphate buffer with a pH value of 5.0. In addition, different solutions can be derived based on it, such as TBS, TBST, TE, TAE, TBE, etc.

Advantages: From the derivative solution, it is known that it has the advantage of being able to prepare buffer solutions with different ranges of acidity and alkalinity using only one buffer system. At the same time, because of its little interference to biological chemical process, it does not precipitate with calcium, magnesium or other heavy metal ions.

3、 Glycine buffer

A typical representative is Bicine, which has a wide pH value and a wide range of applications. Although unlike Tris, which can derive multiple solutions, it also has advantages that cannot be ignored. It can provide a comfortable living environment for cell components and extracts, ensuring stability and not being disturbed by external factors.

As a professional manufacturer of research, development, and sales of various buffer solutions, Desheng specializes in buffer solutions of various specifications and pH values, such as Tris buffer, Bicine buffer, MOPS buffer, CAPS buffer, and other types, suitable for various large-scale experiments. And the company adopts advanced production equipment and has a complete testing system, which can ensure that users do not experience any abnormal situations during use. If you are interested, please click on the website to inquire and purchase!