Application of MOPS buffer in embryo culture

 

MOPS is a widely used biological buffer solution. Its full chemical name is 3-morpholinopropionic acid. CAS number is 1132-61-2, and its appearance is white crystal powder. When it is used, it needs to be prepared into a solution according to a certain proportion, so it has good water solubility and is a commonly used zwitterion buffer solution. MOPS can not only be used for in vitro diagnostic reagents, plant hydroponics, protein purification, electrophoresis analysis, etc., but also for embryo culture.

 

Embryo culture refers to the process of allowing the obtained embryos to grow in artificial culture medium for a period of time after in vitro fertilization. Therefore, it is necessary to control and regulate the pH value of the culture medium to provide a stable environment for embryo growth. MOPS buffer has potential advantages in being used for human oocyte collection and embryo processing, and its buffering ability is less temperature dependent. Its buffering range is between 6.5 and 7.9 pH values, which is closer to the physiological pH value of the human body.

 

The reason why MOPS is suitable for embryo culture is also because its chemical properties are stable, it is not toxic to organisms, and it has no significant permeability to biofilms; When used in experiments such as cell tissue culture or virus diagnosis without reacting with various enzyme preparations or chemical reagents, cells exhibit high vitality. When using, it should be noted that when the concentration is higher than 20mM, it is not suitable for cell culture in mammals.

 

As a zwitterion buffer, MOPS has many advantages, wide applications and large market demand. However, there are very few MOPS manufacturers on the market that can provide stable performance and excellent quality. Hubei Xindesheng Materials Technology Co., Ltd. is a strong manufacturer specializing in the research and development and production of MOPS and other buffering agents, which can ensure that the purity of the products produced can reach over 99%, the absorbance is lower than 0.05, and the process is stable. In addition to MOPS, common biological buffers such as TRIS and HEPES are also highly recognized by users. If you are still struggling with which company to choose as a biological buffer, you may want to try Desheng brand products to ensure that you won't be disappointed!