Why use tris buffer for cell lysis?

 

In biochemical research work, the selection of buffering agents is very cautious. Due to its numerous types, the buffering range of different buffering agents varies. Inadvertently, experimental results may deviate due to incorrect selection or improper use, such as using tris buffering agent instead of other buffering agents in cell lysis. Why is this? Below is a specific introduction for everyone.

 

1、 Buffer range of Tris

As a weakly alkaline solution, its pKa is 8.1 at room temperature of 25 degrees Celsius. According to buffering theory, this also indicates that its effective pH range is between 7.0 and 9.2. The appropriate pH value during cell lysis is 8.0, and using Tris can maintain a stable pH value without any changes.

 

2、 The benefits of using Tris in cell lysis

1. Due to its ability to be used throughout the entire DNA extraction process and its sensitivity to pH values in various external sources of extraction, the use of tris buffer during cell lysis can not only stabilize the acidity and alkalinity, but also ensure the smooth progress of the lysis experiment.

2. General cell lysis is the first step in extracting DNA, completed through a buffer containing tris and ethylenediaminetetraacetic acid, which can help maintain the integrity and stability of the cell membrane.

3. Tris is used in cell lysis experiments. Based on its own performance, it can also interact with LPS (lipopolysaccharide) in the membrane, promoting rapid cell membrane lysis.

 

3、 Precautions for using Tris during cell lysis

1. Although it can stabilize the pH value during the experiment, it is greatly affected by the concentration of the solution. If diluted, the pH value will change. Please note that the dilution behavior should be stopped when the pH value reaches 8.0.

2. Pay attention to the environmental temperature during the experiment. Excessive temperature changes can affect the pH value of Tris, and the buffer range may become unsatisfactory, affecting the experimental effect.

3. Generally, Tris finished products are in powder form, and after being prepared into a solution, they are prone to absorbing CO2 in the air and causing changes. Therefore, it is important to use them immediately during use and not to be exposed to the air for a long time.

 

As a manufacturer of biological buffer solutions, Desheng not only offers Tris, but also sells Caps, Bicine, Mops, Taps, and other products, which can meet customers' different purchasing needs and be applied in different experimental fields. And the purity of the buffer products is over 99%, with stable performance and simple use, which can effectively save time. If you are interested, please feel free to inquire about the details of purchasing!