Tris -- the "shoulder" in biological buffer

 

Tris is a very common biological buffer. Because it is easily soluble in water, strongly alkaline and other excellent characteristics, and it is not easy to react with calcium, magnesium and other heavy metal ions, it is a kind of biological buffer that is widely used in biochemical experiments, and is worthy of the "shoulder" among many biological buffers.

 

Tris has become the backbone of biological buffer solution, not only because it is used in nucleic acid DNA related experimental research, but also in cosmetics industry, virus preservation solution, new paint and other fields. More importantly, tris derived a variety of biological buffers, which also played a great role in biochemical experiments.

 

1. Tris hcl is a kind of acidic solution prepared by appropriate amount of Tris and HCL. It is mainly used in nucleic acid and protein solvents, as well as in electrophoretic buffer, and as a medical intermediate.

 

2. TBS is prepared by appropriate amount of Tris hcl, sodium chloride, potassium chloride, etc. It can be used as the basic reagent of blocking solution in immunohistochemical experiments.

 

3. TBST is a kind of isotonic slow salt solution commonly used in biology, which is prepared by appropriate amount of Tris HCl, sodium chloride and tweet20. It is mainly used in the Western Blot experiment. It should be noted that TBST is easy to volatilize and needs to be sealed for preservation.

 

4. TE solution is prepared by appropriate amount of Tris and EDTA, which is a kind of alkaline electrophoresis buffer. Its main purpose is to dissolve nucleic acids and store DNA and RNA stably.

 

5. TAE buffer solution is prepared by appropriate amount of Tris, acetic acid and EDTA. It is mostly used as the buffer solution for DNA or RNA gel electrophoresis experiments, which is conducive to the migration of DNA molecules and can also prevent precipitation reaction with nucleic acid.

 

6. TBE is prepared by appropriate amount of Tris, boric acid and EDTA. It is an electrophoretic buffer, mainly used in DNA agarose gel electrophoresis experiments.

 

After 17 years of rapid development, Desheng has a huge R&D team and experimental base, specializing in the R&D, production and sales of TRIS and its derivatives as well as a series of common biological buffers such as HEPES and TAPS. It has a wide range of types and uses. If you need to buy, please contact me or check the official website!