Chromatography technology is one of the important tools in modern chemical analysis, widely used in fields such as pharmaceuticals, environmental monitoring, and food safety. The selection of chromatographic columns and packing materials directly affects separation efficiency and resolution. In recent years, the application of Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 in chromatographic column packing materials has gradually attracted attention due to its unique molecular structure and chemical properties. This product has demonstrated significant advantages as a chromatographic column packing material in terms of separation efficiency, column lifespan, selectivity, and durability.

1. Chemical structure and reaction characteristics

Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 is an organochlorosilane with a molecular structure consisting of one octadecyl long chain (C18 chain), two isobutyl groups, and one chlorine group. The specific chemical formula is C26H55ClSi. The Si Cl bond in chlorosilane has high chemical reactivity and can react with the silanol group (Si-OH) of the chromatographic column substrate (such as silica gel) to form hydrolysis resistant silicon oxygen bonds (Si-O-Si), thereby firmly bonding the octadecyl long chain to the surface of the chromatographic column. This chemical modification not only changes the surface properties of the chromatographic column, but also lays the foundation for its application in separating complex samples.

2. Advantages as a filling material for chromatographic columns

2.1) Significant hydrophobicity: suitable for separating non-polar compounds

The molecule of Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 contains an octadecyl long chain (C18), which is extremely hydrophobic and suitable for the separation of non-polar compounds. The enhancement of hydrophobicity means that during the interaction between sample molecules and the stationary phase, non-polar compounds will preferentially interact with the column surface, while polar compounds will be rapidly eluted. This selectivity makes it fillers particularly suitable for separating non-polar or weakly polar compounds, such as fatty acids, alkanes, lipids, etc.

Compared with other short chain modified fillers, C18 long-chain provides longer retention time for analysis, especially when separating complex samples. This longer retention time is of great significance for improving the resolution of non-polar compounds.

2.2) Spatial hindrance effect: reducing residual active sites on the column surface

The two isobutyl groups in Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 provide additional steric hindrance effects. This spatial hindrance not only helps to reduce residual silanol groups on the surface of the chromatographic column, but also effectively prevents the interaction between sample molecules and polar active sites that are not capped. This is particularly important because unsealed silanol groups can easily form strong interactions with polar compounds, leading to peak tailing and poor separation.

By modifying with Diisobutyloctadecylchlorosilane CAS NO.162578-86-1, the surface of the chromatographic column becomes more uniform and hydrophobic, reducing sample retention caused by surface activity. The result is a more symmetrical peak shape and narrower peak width, thereby improving separation efficiency and detection accuracy.

2.3) Chemical stability and durability: suitable for harsh analytical environments

The chromatographic column modified with Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 filling material has excellent chemical stability, especially in the face of harsh environments such as strong acids, strong bases, or organic solvents, exhibiting better durability than other traditional filling materials. The non-polar structure of the C18 chain makes it less susceptible to acid-base hydrolysis or oxidation, thereby extending the service life of the chromatographic column.

In addition, the chromatographic column modified with Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 can maintain its separation performance at high temperatures, which is crucial for gas chromatography (GC) analysis that requires operation at high temperatures. Traditional fillers are prone to degradation or deactivation at high temperatures, while it modified columns can maintain stable performance after multiple heating cycles.

2.4) Chemical Stability and Durability: Chromatographic columns modified with Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 filling materials have excellent chemical stability, especially in harsh environments such as strong acids, bases, or organic solvents, exhibiting better durability than other traditional filling materials. The non-polar structure of the C18 chain makes it less prone to acid-base hydrolysis or oxidation, thereby extending the service life of the chromatographic column. In addition, chromatography columns modified with it can maintain their separation performance at high temperatures, which is crucial for gas chromatography (GC) analysis that requires operation at high temperatures. Traditional fillers are prone to degradation or deactivation at high temperatures, while it modified columns can maintain stable performance after multiple heating cycles.

2.5) Wide range of applications: Separation of polar to non-polar compounds

Although Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 is known for its excellent non-polar separation ability, its chemical structure also allows for a certain degree of separation of polar compounds. The presence of isobutyl provides slight polarity modification for chromatographic columns, allowing them to handle compounds with moderate or weak polarity under certain specific conditions.

In liquid chromatography (HPLC), Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 fillers are particularly suitable for analyzing complex mixtures, including biological samples, drug metabolites, and environmental pollutants. This filling material can not only achieve efficient separation of non-polar compounds, but also handle polar molecules under appropriate mobile phase selection.

2.6) Reducing chromatographic peak tailing: improving separation accuracy and reproducibility

Peak tailing imon problem in chromatographic analysis, especially when dealing with polar samples, where the sample molecules strongly s a cominteract with the unsealed silanol groups, resulting in a broadening of the peak shape and making quantitative analysis difficult. Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 modification can effectively block residual silicon hydroxyl sites, reduce polar interactions, thereby improving peak shape and reducing tailing phenomenon.

Through this modification, the surface of the chromatographic column becomes more uniform, and the interaction between the stationary phase and the mobile phase becomes more consistent, thereby improving the reproducibility and separation accuracy of the experiment. After multiple uses, the chromatographic column modified with Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 can still maintain good separation efficiency, extending the service life of the column.

All in all, Diisobutyloctadecylchlorosilane CAS NO.162578-86-1, as a filling material for chromatography columns, has demonstrated excellent separation performance due to its unique molecular structure and excellent chemical properties. Its strong hydrophobicity, steric hindrance effect, chemical stability, and wide range of applications make it an ideal choice for separating non-polar and weakly polar compounds. By modifying the surface of the chromatographic column, Diisobutyloctadecylchlorosilane CAS NO.162578-86-1 filling material not only improves the chromatographic peak shape and reduces peak tailing, but also extends the service life of the chromatographic column, enhances separation accuracy and reproducibility.

Editor : Alan Liu

Email: alan.liu@dakenchem.com