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International Journal of Biological Macromolecules

International Journal of Biological Macromolecules

IF: 7.7
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Ultrasound-assisted extraction, purification, structural characterization, and hypoglycemic activities of a polysaccharide from Momordica charantia L.

Published:3 December 2024 DOI: 10.1016/j.ijbiomac.2024.138300 PMID: 39638214
Yanhui Zhang,?Haizhao Song,?Jing Lu,?Fang Wang,?Luanfeng Wang,?Ling Xiong,?Xinchun Shen

Abstract

Polysaccharides from Momordica charantia L. (MCP) have attracted interest for their diverse biological activities. This study investigated the ultrasound-assisted extraction of MCP, optimizing conditions using response surface methodology. The optimal extraction parameters were a material-liquid ratio of 1:4 (g/mL), a temperature of 74?°C, an extraction time of 2.4?h, and an ultrasonic power of 296?W, resulting in a total carbohydrate content of 40.22?±?1.69?%, closely matching theoretical predictions. Following extraction, MCP was purified using DEAE Sepharose Fast Flow anion exchange and Sephadex G-100 dextran gel chromatography, isolating a pure polysaccharide fraction, MCPS-3. Structural analysis revealed that MCPS-3, with a molecular mass of 93.796?kDa, consisted of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in molar ratios of 10.66:3.66:258.0:1.0:51.0:9.338. Methylation and NMR analyses suggested that MCPS-3 was a complex polysaccharide, predominantly homogalacturonan domains with rhamnogalacturonan I side chains. In functional assays, MCPS-3 demonstrated inhibition of α-amylase and α-glucosidase activities. Additionally, in insulin-resistant HepG2 cells, MCPS-3 improved glucose consumption, increased glycogen content, and enhanced the activities of hexokinase and pyruvate kinase. These findings provided insights into the structural properties of MCPS-3 and underscored its potential as a natural compound with significant hypoglycemic activity.

Substances (13)

Materials
Procduct Name CAS Molecular Formula Supplier Price
5-Methyl-2-phenyl-1,2-dihydropyrazol-3-one 89-25-8 C10H10N2O 902 suppliers $6.00-$4424.01
Trifluoroacetic acid 76-05-1 C2HF3O2 766 suppliers $9.00-$8444.80
Acarbose 56180-94-0 C25H43NO18 642 suppliers $6.00-$2245.70
Thiazolyl Blue 298-93-1 C18H16BrN5S 401 suppliers $7.00-$1596.00
alpha-Amylase 9000-90-2 NULL 379 suppliers $14.00-$2540.00
2,2-diphenyl-1-picrylhydrazyl 1898-66-4 C18H12N5O6* 260 suppliers $40.00-$1084.20
α-Glucosidase 9001-42-7 NULL 212 suppliers $66.10-$562.00
INSULIN 11070-73-8 C254H377N65O75S6 193 suppliers $72.00-$2660.00
α-Amylase 9000-85-5 NULL 117 suppliers $70.80-$844.00
DEAE SEPHAROSE CL-6B 57407-08-6 80 suppliers $80.00-$3250.00

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