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pECFP-Peroxi 載體,pECFP-Peroxi
  • pECFP-Peroxi 載體,pECFP-Peroxi

pECFP-Peroxi 載體

價(jià)格 990
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2025-01-20
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產(chǎn)品詳情

中文名稱:pECFP-Peroxi 載體英文名稱:pECFP-Peroxi
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2025-01-20 pECFP-Peroxi 載體 pECFP-Peroxi 2ug/990RMB 990 常溫運(yùn)輸 99% 質(zhì)粒/載體

pECFP-Peroxi 載體

質(zhì)粒類型:
熒光蛋白報(bào)告載體
啟動(dòng)子:
CMV
克隆方法:
多克隆位點(diǎn),限制性內(nèi)切酶
載體大小:
4740 bp (查看載體序列)
載體標(biāo)簽:
ECFP
載體抗性:
Kanamycin (卡那霉素)
篩選標(biāo)記:
Neomycin (新霉素)
產(chǎn)品編號(hào)
產(chǎn)品名稱
規(guī)格
價(jià)格
ZY1818
pECFP-Peroxi
2ug
 
pECFP-Peroxi 質(zhì)粒圖譜

pECFP-Peroxi encodes a fusion of the enhanced cyan fluorescent variant of the Aequorea victoria green fluorescent protein gene (GFP) and the peroximal targeting signal 1 (PTS1). The PTS1 sequence is fused to the 3' end of ECFP and encodes the tripeptide SKL, which targets the fusion protein to the matrix of peroxisomes (1–4). The ECFP gene contains six amino acid substitutions. The Tyr-66 to Trp substitution gives ECFP fluorescence excitation (major peak at 433 nm and a minor peak at 453 nm) and emission (major peak at 475 nm and a minor peak at 501 nm) similar to other cyan emission variants (5, 6). The other five substitutions enhance the brightness and solubility of the protein, primarily due to improved protein folding properties and efficiency of chromophore formation (5, 7, 8).

In addition to the chromophore mutations, ECFP contains >190 silent mutations that create an open reading frame comprised almost entirely of preferred human codons (9, 10). Furthermore, upstream sequences flanking ECFP have been converted to a Kozak consensus translation initiation site (11). These changes increase the translational efficiency of the ECFP-PTS1 mRNA and consequently the expression of ECFP-PTS1 in mammalian and plant cells.

The expression of ECFP-PTS1 is driven by the immediate early promoter of CMV (PCMV IE). pECFPPeroxi contains an MCS at the 5' end of the ECFP-PTS1 sequence. Genes of interest cloned into the MCS will be expressed as fusions to the N-terminus of ECFP-PTS1 if they are in the same reading frame as ECFP-PTS1 and there are no intervening stop codons.The vector contains an SV40 origin of replication and a neomycin resistance (Neor) gene for selection (using G418) in mammalian cells. A bacterial promoter upstream of this cassette (P) expresses kanamycin resistance in E. coli. The vector backbone also provides a pUC19 origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

載體應(yīng)用

Expression of ECFP-PTS1 can be used simply for the labeling of peroxisomes. The MCS at the 5' end of ECFP-PTS1 may be used to fuse genes of interest to ECFP-PTS1. The inserted gene should include the initiation ATG codon and be in frame with ECFP-PTS1. The recombinant ECFP vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (12). ECFP can be used for double-or triple-labeling experiments together with EYFP and DsRed using standard fluorescence microscopy and the appropriate filter sets.

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關(guān)鍵字: pECFP-Peroxi 載體/pECFP-Peroxi質(zhì)粒

公司簡介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測試劑等等
成立日期 2016-08-08 (9年) 注冊資本 100萬元整
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主營行業(yè) 生物化工,有機(jī)原料,化學(xué)試劑,醫(yī)藥原料,技術(shù)服務(wù) 經(jīng)營模式 貿(mào)易,試劑,定制,服務(wù)
  • 上海澤葉生物科技有限公司
VIP 6年
  • 公司成立:9年
  • 注冊資本:100萬元整
  • 企業(yè)類型:貿(mào)易
  • 主營產(chǎn)品:主營產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
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