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pECFP-Nuc 載體,pECFP-Nuc
  • pECFP-Nuc 載體,pECFP-Nuc

pECFP-Nuc 載體

價(jià)格 1280
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2025-01-08
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產(chǎn)品詳情

中文名稱:pECFP-Nuc 載體英文名稱:pECFP-Nuc
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2025-01-08 pECFP-Nuc 載體 pECFP-Nuc 2ug/1280RMB 1280 常溫運(yùn)輸 99% 質(zhì)粒/載體

pECFP-Nuc 載體

質(zhì)粒類型:
熒光蛋白報(bào)告載體
啟動(dòng)子:
CMV
載體大小:
4764 bp (查看載體序列)
載體標(biāo)簽:
ECFP
載體抗性:
Kanamycin (卡那霉素)
篩選標(biāo)記:
Neomycin (新霉素)
產(chǎn)品編號(hào)
產(chǎn)品名稱
規(guī)格
價(jià)格
ZY1817
pECFP-Nuc
2ug
 
pECFP-Nuc 質(zhì)粒圖譜

pECFP-Nuc encodes an enhanced cyan fluorescent variant of the Aequorea victoria green fluorescent protein (GFP) gene with three copies of the nuclear localization signal (NLS) of the simian virus 40 large T-antigen fused at its C-terminus (1, 2). The reiteration of the NLS sequence significantly increases the efficiency of translocation of ECFP into the nucleus of mammalian cells (3). The ECFP gene contains six amino acid substitutions. The Tyr-66 to Trp substitution gives ECFP fluorescence excitation (major peak at 433 nm and a minor peak at 453 nm) and emission (major peak at 475 nm and a minor peak at 501 nm) similar to other cyan emission variants (4-6). The five other substitutions enhance the brightness and solubility of the protein, primarily due to improved protein-folding properties and efficiency of chromophore formation (5, 7 & 8).

In addition to the chromophore mutations, ECFP contains >190 silent mutations that create an open reading frame comprised almost entirely of preferred human codons (9). Furthermore, upstream sequences flanking ECFP have been converted to a Kozak consensus translation initiation site (10). These changes increase the translational efficiency of the ECFP mRNA and consequently the expression of ECFP in mammalian and plant cells.

The vector contains an SV40 origin for replication and a neomycin resistance (Neor) gene for selection (using G418) in eukaryotic cells. A bacterial promoter (P) upstream of Neor expresses kanamycin resistance in E. coli. The vector backbone also provides a pUC19 origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production. The ECFP-Nuc vector can be transfected into mammalian cells using any standard transfection method. If desired, stable transfectants can be selected using G418 (11).

載體應(yīng)用

pECFP-Nuc is used for the localized expression of ECFP in the nucleus of mammalian cells. It allows the visualization of the nucleus in living and fixed cells using fluorescence microscopy. ECFP can be used for dual-labeling experiments together with EYFP or EYFP fusion proteins using standard fluorescence microscopy when the appropriate filter sets are applied. Visit here for a complete listing of recommended filter sets. pECFP-Nuc is not meant to be used as a cloning vector. However, unique restriction sites at the 5' end of ECFP, between ECFP and the three copies of the NLS, and at the 3' end of the fusion protein, allow excision or insertion of DNA.

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關(guān)鍵字: pECFP-Nuc 載體/pECFP-Nuc質(zhì)粒

公司簡(jiǎn)介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開(kāi)發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測(cè)試劑等等
成立日期 2016-08-08 (9年) 注冊(cè)資本 100萬(wàn)元整
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬(wàn)以內(nèi)
主營(yíng)行業(yè) 生物化工,有機(jī)原料,化學(xué)試劑,醫(yī)藥原料,技術(shù)服務(wù) 經(jīng)營(yíng)模式 貿(mào)易,試劑,定制,服務(wù)
  • 上海澤葉生物科技有限公司
VIP 6年
  • 公司成立:9年
  • 注冊(cè)資本:100萬(wàn)元整
  • 企業(yè)類型:貿(mào)易
  • 主營(yíng)產(chǎn)品:主營(yíng)產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
  • 公司地址:松江區(qū)新橋鎮(zhèn)賣新公里2077號(hào)8309室
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