Name

Recombinant Murine MIF (Recombinant Murine Migration Inhibitor Factor; rMuMIF)；Recombinant Mouse MIF ; 重組小鼠移動抑制因子

Synonyms

EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MMIF; Phenylpyruvate tautomerase

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Bioassay data are not available.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 1mM DTT.

Endotoxin

Less than 1EU/μg of rMuMIF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Mouse MIF is active on human cells, while human MIF is active on mouse cells. Mouse MIF is 99%, 84%, 90%, and 90%a.a. identical to rat, porcine, bovine and human MIF, respectively.

Amino Acid Sequence

MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTNDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA

產品編號

產品名稱

包裝

KL7320-10μg

Recombinant Murine MIF

10μg

KL7320-50μg

Recombinant Murine MIF

50μg

KL7320-100μg

Recombinant Murine MIF

100μg

KL7320-1mg

Recombinant Murine MIF

1mg

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說明書

1份