Name

Recombinant Human MIA (Recombinant Human Melanoma Inhibitor Activity Protein; rHuMIA)；重組人黑色素瘤活性抑制蛋白

Synonyms

CD-RAP; CD-RAP; melanoma inhibitory activity; Melanoma inhibitory activity protein; melanoma-derived growth regulatory protein

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5μg/ml, corresponding to a specific activity of >200IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 5% Trehalose.

Endotoxin

Less than 0.1EU/μg of rHuMIA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

MIA is an autocrine growth regulatory protein, secreted from chondrocytes and malignant melanoma cells, that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis, as well as a potential marker for rheumatoid arthritis. The MIA is a member of the MIA/OTOR family, which also includes MIA, OTOR, and TANGO, and they share a Src homology-3 (SH3)-like domain. Recombinant Human MIA is a 12.1kDa globular protein containing 107 amino acid residues, including two intramolecular disulfide bonds.

Amino Acid Sequence

GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ

產(chǎn)品編號

產(chǎn)品名稱

包裝

KL7266-5μg

Recombinant Human MIA

5μg

KL7266-20μg

Recombinant Human MIA

20μg

KL7266-100μg

Recombinant Human MIA

100μg

KL266-1mg

Recombinant Human MIA

1mg

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說明書

1份