Name

Recombinant Bovine EK, His-tag (Recombinant Bovine Enterokinase Light Chain, His-tag; rBoEK, His-tag)；重組牛輕鏈腸激酶，His標(biāo)簽

Synonyms

Enterokinase; ENTK; PRSS7; TMPRSS15; EC 3.4.21; EC 3.4.21.9; Enterokinase; ENTKenterokinase; MGC133046; protease, serine, 7 (enterokinase); PRSS7enteropeptidase; Serine protease 7; Transmembrane protease serine 15; transmembrane protease, serine 15

Purity

N/A

Biological Activity

N/A

Physical Appearance

Sterile liquid.

Formulation

50mM Tris-HCl, pH8.0, 0.5M NaCl and 50% glycerol.

Endotoxin

Less than 1EU/μg of rBoEKL, His-tag as determined by LAL method.

Reconstitution

N/A

Category

Enzymes

Background

Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfidelinked 82-140kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35-62kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications. rBoEK with 6X His-tag binds with Ni²⁺ affinity chromatography and was designed for removing from digestion system.

Amino Acid Sequence

N/A

產(chǎn)品編號

產(chǎn)品名稱

包裝

KL7064-100IU

Recombinant Bovine EK, His-tag

100IU

KL7064-250IU

Recombinant Bovine EK, His-tag

250IU

KL7064-1000IU

Recombinant Bovine EK, His-tag

1000IU

-

說明書

1份