Name

Recombinant Protein A/G-Cys (Recombinant Protein A/G-Cys; Protein A/G-Cys)；重組蛋白A/G，C端帶Cys

Synonyms

N/A

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

The recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from additive free solution.

Endotoxin

Less than 0.1EU/μg of Protein A/G-Cys as determined by LAL method.

Reconstitution

Dissolve in distilled water or saline.

Category

Protein A/G

Background

The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C1C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Amino Acid Sequence

NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEC

產品編號

產品名稱

包裝

KL5057-1mg

Recombinant Protein A/G-Cys

1mg

KL5057-5mg

Recombinant Protein A/G-Cys

5mg

KL5057-1g

Recombinant Protein A/G-Cys

1g

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說明書

1份