Name

Recombinant Human p16-INK4a-TAT (Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A, Isoform 1-TAT; rHup16-INK4a-TAT)；重組人細胞周期蛋白依賴激酶抑制劑2A，同工酶1，TAT標簽

Synonyms

ARF; CDK4I; CDKN2A; INK4a; MLM; p14ARF; p16; p19ARF; ARF; CDK4 inhibitor p16-INK4; CDK4IP14ARF; CDKN2; cell cycle negative regulator beta; CMM2P16-INK4A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); INK4; INK4A; INK4a; MLMP16INK4; MTS-1; MTS1P14; Multiple tumor suppressor 1; p14; p14ARF; P16; p16; p16-INK4; p16INK4a; p16INK4A; P16INK4A; p16-INK4a; p19; P19; p19ARF; p19Arf; P19ARF; TP16

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Data is not available.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.0.

Endotoxin

Less than 1EU/μg of rHuP16-INK4a-TAT as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Cyclin-dependent kinase inhibitors (CDKIs) are proteins that bind to and inhibit the activity of CDKs. Two major classes of CDK inhibitors have been identified. The p16 family (p15, p16, p18 and p19) binds to and inhibits the activities of CDK4 and CDK6. The p21 family (p21, p27, p28 and p57) can bind to broad range of CDK-cyclin complexes and inhibit their activities. CDKIs are capable of suppressing growth, and several lines of evidence strongly suggest that at least some CDKIs may be tumor suppressor proteins. p16-INK4A is the member of p16 family and is encoded by CDKN2A gene in humans. It has three isoforms, which are wildly expressed but not detected in brain or skeletal muscle, except that isoform 3 is pancreas-specific. Defects in p16INK4A are a cause of Li-Fraumeni syndrome (LFS) and melanoma-astrocytoma syndrome (MASTS). The TAT transduction peptide can help rHuP16-INK4a with acrossing all kind of biomembranes in vivo and has no effect on the super structure of the protein.

Amino Acid Sequence

EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGRK KRRQRRR

產(chǎn)品編號

產(chǎn)品名稱

包裝

KL7226-5μg

Recombinant Human p16-INK4a-TAT

5μg

KL-7226-25μg

Recombinant Human p16-INK4a-TAT

25μg

KL-7226-100μg

Recombinant Human p16-INK4a-TAT

100μg

KL7226-1mg

Recombinant Human p16-INK4a-TAT

1mg

-

說明書

1份