Name

Recombinant Bovine bFGF (Recombinant Bovine Basic Fibroblast Growth Factor; rBobFGF)；重組牛堿性成纖維細(xì)胞生長(zhǎng)因子

Synonyms

FGF2; FGF-2; FGF2AS; GFG1; HBGH-2; NUDT6; Prostatropin; Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; BFGF; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rBobFGF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Bovine bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Bovine bFGF shares 95% amino acid sequence identity with murine bFGF, and 97% amino acid sequence identity with rat. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. It is also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Amino Acid Sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS

產(chǎn)品編號(hào)

產(chǎn)品名稱

包裝

KL-6443-10μg

Recombinant Bovine bFGF

10μg

KL-6443-50μg

Recombinant Bovine bFGF

50μg

KL-6443-100μg

Recombinant Bovine bFGF

100μg

KL-6443-1mg

Recombinant Bovine bFGF

1mg

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說明書

1份