Name

Recombinant Human IL-2, GMP (Recombinant Human Interleukin-2, GMP; rHuIL-2, GMP)；重組人白細(xì)胞介素2

Synonyms

aldesleukin; Aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH5.0.

Endotoxin

Less than 1EU/μg of rHuIL-2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.

Category

Cytokine

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4⁺ and CD8⁺ T cells, γδ T cells, B cells, dendritic cells and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55kDa IL-2Rα, 75kDa IL-2Rβ, and 64kDa common gamma chain γc/IL-2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56% and 66% amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. Manufactured using all Animal-Free reagents.

Amino Acid Sequence

APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

產(chǎn)品編號(hào)

產(chǎn)品名稱

包裝

KL-7428-5μg

Recombinant Human IL-2, GMP

5μg

KL-7428-100μg

Recombinant Human IL-2, GMP

100μg

KL-7428-1mg

Recombinant Human IL-2, GMP

1mg

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說明書

1份