Name
Recombinant Murine IL-36α, 160a.a. (Recombinant Murine Interleukin-36α, 160a.a.; rMuIL-36α, 160a.a.)；Recombinant Mouse IL-36α, 160a.a. ; 重組小鼠白細(xì)胞介素36α，160a.a.
Synonyms
FIL1 epsilon; IL-1E; IL1F6; IL36A; FIL1; FIL1 epsilon; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL1F6; IL-1F6; IL-1F6 (FIL-1-epsilon); interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rMuIL-36α at 1μg/ml can bind recombinant murine IL-1 Rrp2 with a range of 0.15-5μg/ml.
Physical Appearance
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 5% trehalose.
Endotoxin
Less than 1EU/μg of rMuIL-36α, 160a.a. as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant murine interleukin-36 alpha contains 160 amino acids residues which is a single nonglycosylated polypeptide. Specifically, mouse IL-36α shares 83%a.a. sequence identity with rat IL-36α, 54-60% with human, rabbit, equine and bovine IL-36α.
Amino Acid Sequence
MNKEKELRAA SPSLRHVQDL SSRVWILQNN ILTAVPRKEQ TVPVTITLLP CQYLDTLETN RGDPTYMGVQ RPMSCLFCTK DGEQPVLQLG EGNIMEMYNK KEPVKASLFY HKKSGTTSTF ESAAFPGWFI AVCSKGSCPL ILTQELGEIF ITDFEMIVVH