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DAPI
  • DAPI

DAPI

價(jià)格 758
包裝 1mg
最小起訂量 1mg
發(fā)貨地 上海
更新日期 2020-01-14

產(chǎn)品詳情

英文名稱:DAPI
數(shù)量: 大量供應(yīng)商: 科敏商城
貨號: DAPI英文名: DAPI
規(guī)格: 1?mg保質(zhì)期: 一年
CAS號: IF , F ,保存條件: 低溫
2020-01-14 DAPI DAPI 1mg/758RMB 758

微信關(guān)注 科敏商城 在線客服CST火熱促銷中



品牌:CST,產(chǎn)品名稱:DAPI #4083
英文名稱:DAPI
Product name:DAPI #4083
Applications:IF , F ,
Background: 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).

Directions for Use: DAPI is supplied as a lyophilized powder. For a 20 mg/ml stock, reconstitute the 1 mg in 50 μl deionized water or dimethylformamide. Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:

Immunofluorescence: Counterstain with DAPI as the final step in your staining procedure. Rinse samples twice in PBS for five min each. Dilute DAPI stock solution to a concentration between 1 - 0.1 μg/ml in PBS and incubate for 5 min at room temperature in the dark. Rinse samples once in PBS and then prepare for imaging. Examine immediately using appropriate excitation wavelength.

Alternatively, dilute DAPI stock solution to a concentration between 1 - 0.1 μg/ml in mounting media, apply to cells, and prepare for imaging. Examine immediately using appropriate excitation wavelength.

Flow Cytometry: Rinse samples once in Incubation Buffer. Dilute DAPI stock solution to a concentration between 1.60-0.0125 μg/ml in PBS and incubate for 15 min at room temperature in the dark before analyzing cells on flow cytometer.

Storage:

Storage lyophilized or in solution at 4°C, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 2-3 weeks to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description:

DAPI is supplied as a lyophilized powder in 1 mg units. It can be used to examine cellular DNA in fluorescent microscopy and cytometry applications.

Molecular Formula:

C16H15N5 2(HCl)

科研采購盡在科敏商城

溫馨提示:不可用于臨床治療。
關(guān)鍵字: DAPI;試劑

公司簡介

成立日期 2011-12-05 (13年) 注冊資本 50萬人民幣
員工人數(shù) 年?duì)I業(yè)額
主營行業(yè) 經(jīng)營模式
  • 上海恒斐生物科技有限公司
非會(huì)員
  • 公司成立:13年
  • 注冊資本:50萬人民幣
  • 企業(yè)類型:董艷民
  • 主營產(chǎn)品:
  • 公司地址:上海市閔行區(qū)景聯(lián)路439號4幢A區(qū)410
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