乙酸鈉(NaAc)又稱醋酸鈉。固體為無(wú)色透明結(jié)晶或白色顆粒。常溫下為三水化合物,120度時(shí)失去結(jié)晶水,相對(duì)密度為:1.45(三水化合物)。分子量為136.08(三水化合物);
本產(chǎn)品為3M的乙酸鈉溶液,pH為5.2。
配制方法:
3 M 乙酸鈉(pH=5.2)的配制:在800ml水中溶解408.1g三水乙酸鈉,用冰醋酸調(diào)節(jié)pH值至5.2,加水定容至1L,分裝后高壓。
用途:乙酸鈉可用于測(cè)定鉛、銅、鎳和鐵等,也是一種常用的緩沖劑。在核酸提取中,加入乙酸鈉緩沖液,可調(diào)節(jié)鹽離子的濃度,中和核酸分子的負(fù)電荷,便于加入乙醇后使DNA沉淀下來(lái)。
Sodium acetate buffer solution as the conjugate base of acetic acid, a solution of sodium acetate and acetic acid can act as a buffer to keep a relatively constant pH level. This is useful especially in biochemical applications where reactions are pH-dependent in a mildly acidic range (pH 4-6).
Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay and in vitro enzyme inhibition assay.
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