| 產品編號 |
產品名稱 |
產品包裝 |
產品價格 |
| AF1432 |
Met (C-Met) Rabbit Monoclonal Antibody |
50μl |
618.00元 |
|
來源
|
用途
|
交叉反應性
|
分子量
|
|
Rabbit
|
WB, IF, IHC, ICC, FC
|
H
|
155.0kDa
|
???????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
???????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
???????配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
???????建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
-
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
1:50-1:100
|
-
|
-
|
抗體詳細信息如下:
|
About this Antibody
|
|
Name
|
Met (C-Met) Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
Affinity purification
|
|
About the Immunogen
|
|
Immunogen
|
Recombinant protein
|
|
Gene ID
|
4233(Human)
|
|
SwissProt
|
P08581(Human)
|
|
Synonyms
|
AUTS9; HGFR; RCCP2; c-Met
|
|
Category
|
Protein Tyrosine Kinase
|
|
Background
|
The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines . The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
|
包裝清單:
|
產品編號
|
產品名稱
|
包裝
|
|
AF1432
|
Met (C-Met) Rabbit Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
???????Met (C-Met) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
???????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
???????回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
???????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
???????本產品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
???????為了您的安全和健康，請穿實驗服并戴一次性手套操作。