價(jià)格 | 詢價(jià) |
包裝 | 1mg |
最小起訂量 | 1mg |
發(fā)貨地 | 上海 |
更新日期 | 2025-01-30 |
中文名稱:Recombinant Equine IFN-γ | 英文名稱:Recombinant Equine IFN-γ |
有效期: 一年 | 產(chǎn)品類別: 重組蛋白 細(xì)胞因子 |
產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 產(chǎn)品包裝 | 產(chǎn)品價(jià)格 |
P6483-1mg | Recombinant Equine IFN-γ | 1mg | 6794.00元 |
Species | Gene ID | Accession | Source | Length | MW | Tag |
Equine | 100034181 | P42160 | E. coli | 143aa | 16.7kDa | - |
About this Protein | |
Name | Recombinant Equine IFN-γ (Recombinant Equine Interferon-gamma; rEqIFN-γ);重組馬干擾素γ |
Synonyms | IFNG; IFG; IFI; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma |
Purity | >97% by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis (EMC) virus is less than 10.0ng/ml, corresponding to a specific activity of >1.0×105IU/mg. |
Physical Appearance | Sterile Filtered lyophilized (freeze-dried) liquid-like powder. |
Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, with 5% trehalose. |
Endotoxin | Less than 0.1EU/μg of rEqIFN-γ as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Cytokine |
Background | Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by Tlymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation. Equine IFN-γ shares 73%~82% amino acid sequence identity with bovine, canine, feline, and porcine IFN-γ and 42%~64% with cotton rat, human,murine, rat, and rhesus macaque IFN-γ. |
Amino Acid Sequence | QAAFFKEIEN LKEYFNASNP DVGDGGPLFL DILKNWKEDS DKKIIQSQIV SFYFKLFENL KDNQVIQKSM DTIKEDLFVK FFNSSTSKLE DFQKLIQIPV NDLKVQRKAI SELIKVMNDL SPKANLRKRK RSQNPFRGRR ALQ |
包裝清單:
產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 包裝 |
P6483-20μg | Recombinant Equine IFN-γ | 20μg |
P6483-100μg | Recombinant Equine IFN-γ | 100μg |
P6483-1mg | Recombinant Equine IFN-γ | 1mg |
- | 說(shuō)明書(shū) | 1份 |
保存條件:
???????-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活,所以首次配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液),須分裝后-20℃或更低溫度凍存,以避免反復(fù)凍融。
注意事項(xiàng):
???????由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn),使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
???????本產(chǎn)品在凍干時(shí)添加了海藻糖作為保護(hù)劑,此時(shí)凍干后本產(chǎn)品看起來(lái)仍然像是液體,屬于正?,F(xiàn)象。在凍干工藝上,海藻糖作為常用的凍干保護(hù)劑,可顯著阻止蛋白質(zhì)二級(jí)結(jié)構(gòu)的改變,防止凍干過(guò)程中的蛋白變性。
???????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
???????為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
成立日期 | 2007-07-19 (18年) | 注冊(cè)資本 | 1000.000000萬(wàn)人民幣 |
員工人數(shù) | 500人以上 | 年?duì)I業(yè)額 | ¥ 500萬(wàn)-1000萬(wàn) |
主營(yíng)行業(yè) | 生化試劑,核苷,核苷酸,寡核苷酸,抗體,蛋白組學(xué),生物活性小分子 | 經(jīng)營(yíng)模式 | 試劑 |
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