Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

16153

P18893

E. coli

160aa

18.7kDa

－

About this Antibody

Name

Recombinant Murine IL-10 (Recombinant Murine Interleukin-10; rMuIL-10)；Recombinant Mouse IL-10 ;?重組小鼠白細(xì)胞介素10

Synonyms

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1ng/ml, corresponding to a specific activity of >1.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuIL-10 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HCl to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2). The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Rα/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature murine IL-10 shares 70%-77% amino acid sequence identity with human, bovine, porcine IL-10, but murine IL-10 does not act on human cells.

Amino Acid Sequence

SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS

產(chǎn)品編號(hào)

產(chǎn)品名稱

包裝

P5939-2μg

Recombinant Murine IL-10

2μg

P5939-10μg

Recombinant Murine IL-10

10μg

P5939-100μg

Recombinant Murine IL-10

100μg

P5939-1mg

Recombinant Murine IL-10

1mg

-

說明書

1份