Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

16189

P07750

E. coli

121aa

13.5kDa

－

About this Antibody

Name

Recombinant Murine IL-4 (Recombinant Murine Interleukin-4; rMuIL-4)；Recombinant Mouse IL-4 ;?重組小鼠白細(xì)胞介素4

Synonyms

BCDF; BSF1; B cell growth factor 1; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; Binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra; pitrakinra

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2ng/ml, corresponding to a Specific Activity of >5×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuIL-4 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4⁺ T-cells into Th2 cells. IL-4 enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The mouse IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds and contains 121 amino acids residues which is a single non-glycosylated polypeptide. The human IL-4 shares about 40%a.a. sequence identity with mouse/rat IL-4 and they are species-specific in their activities.

Amino Acid Sequence

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

產(chǎn)品編號(hào)

產(chǎn)品名稱(chēng)

包裝

P5916-5μg

Recombinant Murine IL-4

5μg

P5916-20μg

Recombinant Murine IL-4

20μg

P5916-100μg

Recombinant Murine IL-4

100μg

P5916-1mg

Recombinant Murine IL-4

1mg

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說(shuō)明書(shū)

1份