Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

18413

P53347

E. coli

181aa

20.4kDa

－

About this Antibody

Name

Recombinant Murine OSM (Recombinant Murine Oncostatin-M; rMuOSM)；Recombinant Mouse OSM ;?重組小鼠腫瘤抑制素

Synonyms

MGC20461; oncostatin M; oncostatin-M

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 5% trehalose.

Endotoxin

Less than 1EU/μg of rMuOSM as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 181 amino acids and has a molecular mass of 20.4kDa. It has approximately 48% and 72% amino acid sequence identity with human and rat OSM.

Amino Acid Sequence

NRGCSNSSSQ LLSQLQNQAN LTGNTESLLE PYIRLQNLNT PDLRAACTQH SVAFPSEDTL RQLSKPHFLS TVYTTLDRVL YQLDALRQKF LKTPAFPKLD SARHNILGIR NNVFCMARLL NHSLEIPEPT QTDSGASRST TTPDVFNTKI GSCGFLWGYH RFMGSVGRVF REWDDGSTRS R

產(chǎn)品編號

產(chǎn)品名稱

包裝

P6038-2μg

Recombinant Murine OSM

2μg

P6038-10μg

Recombinant Murine OSM

10μg

P6038-100μg

Recombinant Murine OSM

100μg

P6038-1mg

Recombinant Murine OSM

1mg

-

說明書

1份