Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

21832

P40226

E. coli

174aa

18.7kDa

－

About this Antibody

Name

Recombinant Murine TPO (Recombinant Murine Thrombopoietin; rMuTPO)；Recombinant Mouse TPO ;?重組小鼠促血小板生成素

Synonyms

MGDF; MK-CSF; THPO; Tpo; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDFC-mpl ligand; ML; MPL ligand; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; thrombopoietin; thrombopoietin nirs variant 1; TPOMKCSF

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 5% Trehalose.

Endotoxin

Less than 0.1EU/μg of rMuTPO as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Thrombopoietin (TPO), the ligand for the receptor encoded by the c-Mpl proto-oncogene, is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. The cDNAs for TPO have recently been cloned from canine, murine and human sources. The proteins from these three species are highly conserved, exhibiting from 69-75% sequence identity at the amino acid level. Two distinct domains, separated by a pair of arginine residues that may be a proteolytic cleavage site, have been identified in TPO: the amino terminal region exhibiting sequence homology to erythropoietin and the carboxy terminal region containing multiple potential N-linked glycosylation sites. Recombinant TPO has now been shown to stimulate the maturation, as well as the proliferation, of megakaryocytes and may have important therapeutic applications for the treatment of various clinical conditions associated with thrombocytopenia.

Amino Acid Sequence

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

產(chǎn)品編號

產(chǎn)品名稱

包裝

P6011-2μg

Recombinant Murine TPO

2μg

P6011-10μg

Recombinant Murine TPO

10μg

P6011-100μg

Recombinant Murine TPO

100μg

P6011-1mg

Recombinant Murine TPO

1mg

-

說明書

1份