Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

25542

P50229

E. coli

69aa

7.9kDa

－

About this Antibody

Name

Recombinant Rat MIP-1α/CCL3 (Recombinant Rat Macrophage Inflammatory Protein-1α/CCL3; rRtMIP-1α/CCL3)；重組大鼠巨噬細胞炎癥蛋白1α/CCL3

Synonyms

LD78a; MIP-1 alpha; C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 10-100ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

Endotoxin

Less than 0.1EU/μg of rRtMIP-1α/CCL3 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Chemokines

Background

CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant rat MIP-1α is a 7.9kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and mature rat CCL3 shares 74%, 91% and 88% amino acid sequence identity with human, mouse and cotton rat CCL3, respectively.

Amino Acid Sequence

APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA

產(chǎn)品編號

產(chǎn)品名稱

包裝

P6906-5μg

Recombinant Rat MIP-1α/CCL3

5μg

P6906-20μg

Recombinant Rat MIP-1α/CCL3

20μg

P6906-100μg

Recombinant Rat MIP-1α/CCL3

100μg

P6906-1mg

Recombinant Rat MIP-1α/CCL3

1mg

-

說明書

1份