Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8. DNase I is activated by bivalent metals, and inhibited by chelating agents such EDTA and sodium dodecyl sulfate. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.
Deoxyribonuclease of Enzymeking Biotechnoogy Co., Ltd. has been chromatographically purified to remove trace contaminating proteases. DNase may be used in clinic to thin and liquify mucus in the lungs.
Enzymeking Biotechnology Co., Ltd.
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