EGFR(L861Q)  [100 μL] Catalog Number:  CR-M26077 Gene Symbol:  EGFR/HER1/ERBB1 Description:   Anti EGFR(L861Q) Mouse Monoclonal Antibody Background:    EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of L861Q, human origin. Tested Applications:   ELISA, WB, IF, IHC Recommended Dilutions:    ELISA: 1:1000-1:5000  WB: 1:1000-1:5000  IHC: 1:50-1:200 Concentration:    1 mg/ml Host:   Mouse Format:   Liquid Clonality:   Monoclonal Isotype:    Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Recognizes EGFR(L861Q), but not wild type EGFR from vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles. Western blot:

Western blot analysis of recombinant EGFR(L861Q) and wildtype proteins. Purified His-tagged EGFR(L861Q) protein (amino acids 702-987, lane 2, Cat. # CR-P10211) and corresponding wild type protein (lane 1, Cat. # CR-P10210) were blotted with anti-EGFR(L861Q) mouse monoclonal antibody (Cat. # CR-M26077). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti EGFR(L861Q) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(L861Q) plasmid (right column), then fixed and stained with anti- EGFR(L861Q) monoclonal antibody (Cat. #CR-M26077).