"MIA PaCa-2人胰腺癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

傳代比例：1:2-1:4(首次傳代建議1:2)

生長特性：貼壁生長

當(dāng)T25瓶復(fù)蘇細(xì)胞收到貨時(shí)，請觀察好細(xì)胞狀態(tài)后，將T25細(xì)胞瓶壁進(jìn)行75%酒精消毒，將T25瓶置于37度培養(yǎng)箱放置2-4h，以便穩(wěn)定細(xì)胞狀態(tài)，當(dāng)細(xì)胞密度達(dá)80%-90%，即可進(jìn)行首次傳代培養(yǎng)；干冰運(yùn)輸?shù)募?xì)胞凍存管收到貨后，需立即轉(zhuǎn)入液氮保存或直接進(jìn)行復(fù)蘇(第三天換液并檢查復(fù)蘇細(xì)胞密度，以便進(jìn)行下一步)。 能夠在實(shí)驗(yàn)室條件下進(jìn)行大量培養(yǎng)和繁殖。這種細(xì)胞系在分子生物學(xué)和生物技術(shù)研究中十分常用。

換液周期：每周2-3次

TCCPAN2 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HLEC細(xì)胞、QGP-1細(xì)胞、OP9細(xì)胞

SU-DHL1 Cells;背景說明：間變性大細(xì)胞淋巴瘤；胸腔積液轉(zhuǎn)移；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hs695細(xì)胞、HEC1B細(xì)胞、Mono-Mac-6細(xì)胞

A101D Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：４-１：６傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：SNU-C2B細(xì)胞、NCI-A549細(xì)胞、B6Tert-1細(xì)胞

MIA PaCa-2人胰腺癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

背景信息：詳見相關(guān)文獻(xiàn)介紹

┈訂┈購(技術(shù)服務(wù))┈熱┈線：1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同號】┈Q┈Q：3┈1┈8┈0┈8┈0┈7┈3┈2┈4；

公司細(xì)胞系主要引進(jìn)ATCC、DSMZ、JCRB、KCLB、RIKEN、ECACC等細(xì)胞庫，細(xì)胞系體外培養(yǎng)，它們會成長為單層細(xì)胞，附著或緊貼在培養(yǎng)瓶上，或懸浮在體外的溶液中，細(xì)胞系復(fù)蘇周期短，公司細(xì)胞系狀態(tài)良好，飽滿，有光澤等優(yōu)點(diǎn)。EDTA的作用：許多人不用胰酶，只用EDTA，或者用胰酶/EDTA聯(lián)合作用。這里要明白，胰酶切割細(xì)胞外基質(zhì)的一些負(fù)責(zé)粘連和附著的蛋白，而EDTA通過螯合Ca離子，作用于整聯(lián)蛋白的活性，所以EDTA的作用更加溫和。有的人在胰酶里添加一些EDTA，或者對付特別難消化的細(xì)胞，添加多一些EDTA，就是這個(gè)道理。一般不要試圖延長消化時(shí)間(如果10min還消化不下來的話)，而應(yīng)該想其它辦法。

產(chǎn)品包裝：復(fù)蘇發(fā)貨：T25培養(yǎng)瓶(一瓶)或凍存發(fā)貨：1ml凍存管(兩支)

來源說明：細(xì)胞主要來源ATCC、ECACC、DSMZ、RIKEN等細(xì)胞庫

MIA PaCa-2人胰腺癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

物種來源：人源、鼠源等其它物種來源

C6661 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：MOLP-8細(xì)胞、THLE-3細(xì)胞、NS1細(xì)胞

SK-RC 52 Cells;背景說明：腎癌；縱隔膜轉(zhuǎn)移;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：BLO11細(xì)胞、McCoy細(xì)胞、Vertebral Cancer of the Prostate細(xì)胞

Jurkat (clone E6-1) Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SW756細(xì)胞、CEM-CCRF (CAMR)細(xì)胞、MOLT-16細(xì)胞

K7M2 wt Cells;背景說明：骨肉瘤；肺轉(zhuǎn)移；雌性；BALB/c;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Caov-4細(xì)胞、J-774A.1細(xì)胞、Jeko1細(xì)胞

┈訂┈購(技術(shù)服務(wù))┈熱┈線：1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同號】┈Q┈Q：3┈1┈8┈0┈8┈0┈7┈3┈2┈4；

形態(tài)特性：上皮細(xì)胞樣

細(xì)胞培養(yǎng)無菌操作步驟和注意事項(xiàng)：１.打開無菌工作臺及凈化室的紫外燈，消毒半小時(shí)以上。２.進(jìn)入凈化室前先關(guān)閉紫外燈，打開超凈臺風(fēng)機(jī)，等待３０min以上，以排盡臭氧。３.穿HAO隔離衣，戴HAO口罩，帽子。４.風(fēng)淋２分鐘。５.０.１%水泡手，擦干。６.點(diǎn)燃酒精燈;超凈臺內(nèi)應(yīng)避免放入過多的物品;使用的吸管，滴管，試管，培養(yǎng)瓶等均事先滅菌。７.打開各類瓶蓋前先過火，以固定灰塵;打開的瓶口、試管口過火焰，鑷子使用前應(yīng)經(jīng)火焰燒灼。８.水平式風(fēng)機(jī)的超凈臺，應(yīng)使瓶口斜置，應(yīng)盡量避免瓶口敞開直立。９.同一根吸管或滴管不應(yīng)連續(xù)用于幾個(gè)不同的細(xì)胞系；吸取培養(yǎng)基的吸管應(yīng)離開培養(yǎng)瓶或試管口０.５cm，避免伸入培養(yǎng)瓶口或試管口；以防止細(xì)胞系的互相混雜污染。１０.漏在培養(yǎng)瓶上或臺上的體，立即用酒精棉球擦凈。１１.操作完畢后恢復(fù)工作臺面。

RGC5 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HeLa.S3細(xì)胞、L- cell細(xì)胞、SW839細(xì)胞

ID8 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：IPI-2I細(xì)胞、RA 1細(xì)胞、FHs 74 Int細(xì)胞

624 MEL Cells;背景說明：黑色素瘤；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SNUC1細(xì)胞、CCD-841CoN細(xì)胞、COLO-684細(xì)胞

NCIH548 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：COLO 679細(xì)胞、HGC27細(xì)胞、NCI-H838細(xì)胞

Plaepi 34 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：MDA PCa 2b細(xì)胞、NBL_S細(xì)胞、CA922細(xì)胞

H2141 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：３-４天換液１次。;生長特性：懸浮生長;形態(tài)特性：聚團(tuán)懸浮;相關(guān)產(chǎn)品有：Reuber H-35細(xì)胞、LP-1細(xì)胞、NCI-Hut 125細(xì)胞

Panc 3.27 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：J774細(xì)胞、P3JHR-1細(xì)胞、HS852.T細(xì)胞

GM03573A Cells;背景說明：這是P３X６３Ag８(ATCCTIB-９)的一個(gè)不分泌克隆。Kappa鏈合成了但不分泌。能抗０.１mM８-氮雜鳥嘌呤但不能在HAT培養(yǎng)基中生長。據(jù)報(bào)道它是由于缺失了３-酮類固醇還原酶活性的膽固醇營養(yǎng)缺陷型。檢測表明肢骨發(fā)育畸形病毒(鼠痘)陰性。;傳代方法：１：２傳代，３天內(nèi)可長滿。;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞;相關(guān)產(chǎn)品有：Panc 5.04細(xì)胞、NOMO1細(xì)胞、LLC-PK1細(xì)胞

LO2 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：IOSE 29細(xì)胞、NR 8383細(xì)胞、Hs 695T細(xì)胞

143B Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２—１：５傳代；每周換液２-３次;生長特性：貼壁生長;形態(tài)特性：混合型;相關(guān)產(chǎn)品有：JVM2細(xì)胞、Hs-695-T細(xì)胞、V 79-4細(xì)胞

RPMI 7951 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：４傳代，２天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：X63.Ag8.653細(xì)胞、BEAS 2B細(xì)胞、SU-DHL-4細(xì)胞

Y1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CaSki細(xì)胞、28SC細(xì)胞、LS-123細(xì)胞

HPBALL Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HBL100細(xì)胞、FDCP1細(xì)胞、3T3-F442A細(xì)胞

Be-Wo Cells;背景說明：取自人絨癌腦轉(zhuǎn)移組織，在倉鼠頰囊移植傳代８年。利用移植瘤組織進(jìn)行體外培養(yǎng)，建立細(xì)胞系。利用不同傳代方法建立了不同亞系，JEG-３是其衍生克隆。該細(xì)胞可以產(chǎn)生雌激素、孕激素、雌酮、雌二醇、雌三醇、hCG、胎盤催乳素、角蛋白。;傳代方法：１：３傳代，３-４天換液一次;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：BC-022細(xì)胞、SIRC細(xì)胞、3T3(A31)細(xì)胞

PF-382 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞;相關(guān)產(chǎn)品有：HuT-102細(xì)胞、Hs888 Lu細(xì)胞、SNU-398細(xì)胞

Medical University of Graz-Chordoma 1 Cells;背景說明：骶骨脊索瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HEH2細(xì)胞、CA46細(xì)胞、B95-8細(xì)胞

Centre Antoine Lacassagne-51 Cells;背景說明：乳腺癌；胸腔積液轉(zhuǎn)移；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LWnt-3A細(xì)胞、HT 1197.T細(xì)胞、SRA01/04細(xì)胞

Abcam HEK293T RRAGA KO Cells(提供STR鑒定圖譜)

AG12502 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line RRB168 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XE404 Cells(提供STR鑒定圖譜)

BY00332 Cells(提供STR鑒定圖譜)

CS97iHD180-n1 Cells(提供STR鑒定圖譜)

DA04456 Cells(提供STR鑒定圖譜)

DD3420 Cells(提供STR鑒定圖譜)

GM02408 Cells(提供STR鑒定圖譜)

hEM15A Cells;背景說明：子宮內(nèi)膜間質(zhì)細(xì)胞；永生化；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-H2591細(xì)胞、U-251_MG細(xì)胞、M-14細(xì)胞

MIA PaCa-2人胰腺癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

LX-2 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３傳代，２-３天換液一次;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MDA-MB-435S細(xì)胞、OVCA433_Bast細(xì)胞、GM04678細(xì)胞

HL7702 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HPAFII細(xì)胞、MCAEC細(xì)胞、H-1373細(xì)胞

FAT Cells;背景說明：鼻咽癌;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Dunn LM8細(xì)胞、IPLB-SF 21AE細(xì)胞、H1650_CO細(xì)胞

8305C Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：６傳代;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MKN28細(xì)胞、NCI-H719細(xì)胞、SK-MEL2細(xì)胞

SKML-28 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：８傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：星形的;相關(guān)產(chǎn)品有：CT26.CL25細(xì)胞、TE32細(xì)胞、PG13細(xì)胞

4C11 [Mouse hybridoma against human FcRn] Cells(提供STR鑒定圖譜)

OVMANA Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：５ x １０^４ cells/ml;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：SK-MEL-5細(xì)胞、LIXC-002細(xì)胞、PA317細(xì)胞

KP-N-NS Cells;背景說明：源自腦轉(zhuǎn)移灶的腎上腺神經(jīng)母細(xì)胞瘤。;傳代方法：１：２傳代;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CV 1細(xì)胞、NTERA-2cl.D1細(xì)胞、OVCAR420細(xì)胞

MonoMac6 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞;相關(guān)產(chǎn)品有：DMS53細(xì)胞、SUM190細(xì)胞、NCI-H810細(xì)胞

ATDC-5 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：消化３-５分鐘，１：２,３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：KMST-6細(xì)胞、OVCAR-3細(xì)胞、MBMEC細(xì)胞

IMR-90 Cells;背景說明：W.W. Nichols及其同事從一位１６周女嬰的肺中取材，建立了人二倍體成纖維細(xì)胞株IMR-９０。分裂潛能，病毒感受性和其它性質(zhì)都得到了充分研究，因而這株細(xì)胞可以作為WI-３８或其它標(biāo)準(zhǔn)人肺細(xì)胞株的替代株。有報(bào)道稱這株細(xì)胞在表現(xiàn)出衰老前可倍增５８次。;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LC1細(xì)胞、WILL2細(xì)胞、MIN6細(xì)胞

CP-70 Cells;背景說明：卵巢癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MHCC97-H細(xì)胞、SNU-5細(xì)胞、UMC-11細(xì)胞

MRMT-1 Cells;背景說明：乳腺癌；SD;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HOEC細(xì)胞、SNU-638細(xì)胞、CORL88細(xì)胞

Saos2 Cells;背景說明：該細(xì)胞是FoghJ和TrempeG分離和鑒定的眾多人類腫瘤細(xì)胞系中的一種；該細(xì)胞來自一位１１歲的白人女性的骨肉瘤組織?；颊呓?jīng)過放療以及甲喋呤、阿霉素、長春新堿、環(huán)磷酰胺和aramycin-C等多種藥物治療。該細(xì)胞在免疫抑制小鼠中不致瘤，細(xì)胞表達(dá)表皮生長因子EGF受體、轉(zhuǎn)化生長因子β（１型和２型）受體。;傳代方法：１：２-１：４傳代；每周１-２次。;生長特性：貼壁生長;形態(tài)特性：上皮樣；多角形;相關(guān)產(chǎn)品有：BHK21細(xì)胞、CL MC/9細(xì)胞、RH-30細(xì)胞

GM22565 Cells(提供STR鑒定圖譜)

HAP1 MFSD8 (-) 2 Cells(提供STR鑒定圖譜)

HT(H9) Cells;背景說明：H９細(xì)胞是HUT７８（ATCCTIB１６１）的克隆系（Callo，RC，etal）。細(xì)胞表面帶有CD３、CD４標(biāo)記。研究表明，該細(xì)胞系對人體免疫缺陷病毒（HIV-１）敏感，可用于檢測、分離和增殖HIV-１，也可用于其它人類Tcell病毒的研究。;傳代方法：１：３傳代,２-３天傳一代;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：Jurkat E6-1細(xì)胞、PLA-801C細(xì)胞、CG-4細(xì)胞

CCD 1112SK Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：OVCAR-432細(xì)胞、OV1-P細(xì)胞、H-1650細(xì)胞

H2073 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：６傳代 ;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：253J B-V細(xì)胞、CSQT-2細(xì)胞、McA-RH8994細(xì)胞

SUPT-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：２-３天換液１次。;生長特性：懸浮生長;形態(tài)特性：淋巴母細(xì)胞樣　;相關(guān)產(chǎn)品有：RIN14B細(xì)胞、Hs852T細(xì)胞、Cates 1B細(xì)胞

UCD-MLA-144 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SW-900細(xì)胞、L132細(xì)胞、SK MEL 5細(xì)胞

SK-ES1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：５傳代；每周換液２-３次;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：HS-5細(xì)胞、PSN1細(xì)胞、PFSK1細(xì)胞

MARC 145 Cells;背景說明：胚腎；自發(fā)永生;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CoCL2細(xì)胞、CP70細(xì)胞、T-T細(xì)胞

HCE-T Cells;背景說明：角膜上皮細(xì)胞；Ad-SV４０轉(zhuǎn)化；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Panc-8_13細(xì)胞、CBRH-7919細(xì)胞、HTh-74細(xì)胞

HPSI1116i-aimh_5 Cells(提供STR鑒定圖譜)

KCB 88002 Cells(提供STR鑒定圖譜)

MG1.19 Cells(提供STR鑒定圖譜)

NIMHi009-A Cells(提供STR鑒定圖譜)

RAW 264.7 Rab29 KO Cells(提供STR鑒定圖譜)

Ubigene HEK293 WDR24 KO Cells(提供STR鑒定圖譜)

WMUi023-A Cells(提供STR鑒定圖譜)

HeLa S3 ATF4 KO Cells(提供STR鑒定圖譜)

H322 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：P3-x63-Ag8 653細(xì)胞、Jiyoye細(xì)胞、293F細(xì)胞

WERI-Rb 1 Cells;背景說明：WERI-Rb-I細(xì)胞株是１９７４年R.M. McFall 和 T.W. Sery建立的兩株人眼癌細(xì)胞系中的一株。 細(xì)胞能在Difco Bacto-Agar中存活但不形成克隆。 掃描電鏡顯示在表面囊泡，板狀偽足和微絨毛在數(shù)量上和頻率上的改變。 細(xì)胞分化研究，腫瘤治療的動物模型和生化評價(jià)都涉及這株細(xì)胞。;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿。;生長特性：貼壁生長;形態(tài)特性：圓形細(xì)胞聚集成葡萄狀;相關(guān)產(chǎn)品有：C4-2細(xì)胞、RK13細(xì)胞、Y3-Ag1,2,3細(xì)胞

CD18 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LUDLU 1細(xì)胞、MARC-145細(xì)胞、HCC0827細(xì)胞

NCI-H165 Cells;背景說明：非小細(xì)胞肺癌;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H69C細(xì)胞、MC-4細(xì)胞、HPF細(xì)胞

NCI-H716 Cells;背景說明：從一位經(jīng)５-尿嘧啶治療的患者腹水中得到的細(xì)胞建立了這個(gè)細(xì)胞株。 與其它結(jié)直腸癌細(xì)胞系不同，這株細(xì)胞有多巴脫羧酶，細(xì)胞質(zhì)中有核心致密的內(nèi)分泌型顆粒。 這株細(xì)胞不表達(dá)TAG-７２ 或CA１９-９抗原，也不生成癌胚抗原（CEA）;傳代方法：１：３—１：６傳代，每周換液２—３次;生長特性：懸浮生長，聚團(tuán)，少數(shù)貼壁;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：GM 637細(xì)胞、NT2-D1細(xì)胞、PK13細(xì)胞

NCI-H716 Cells;背景說明：從一位經(jīng)５-尿嘧啶治療的患者腹水中得到的細(xì)胞建立了這個(gè)細(xì)胞株。 與其它結(jié)直腸癌細(xì)胞系不同，這株細(xì)胞有多巴脫羧酶，細(xì)胞質(zhì)中有核心致密的內(nèi)分泌型顆粒。 這株細(xì)胞不表達(dá)TAG-７２ 或CA１９-９抗原，也不生成癌胚抗原（CEA）;傳代方法：１：３—１：６傳代，每周換液２—３次;生長特性：懸浮生長，聚團(tuán)，少數(shù)貼壁;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：GM 637細(xì)胞、NT2-D1細(xì)胞、PK13細(xì)胞

NW-MEL-38 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LL/2(LLc1)細(xì)胞、D324 Med細(xì)胞、NCIH1944細(xì)胞

NCI H69 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２—１：４傳代，每周換液２次;生長特性：懸浮生長，聚團(tuán);形態(tài)特性：聚團(tuán)懸浮;相關(guān)產(chǎn)品有：SU.86.86細(xì)胞、CAL51細(xì)胞、SNU-387細(xì)胞

H-1755 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCIH2342細(xì)胞、COLO-680N細(xì)胞、OCI-AML-3細(xì)胞

ATN1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NOR 10細(xì)胞、GM-215細(xì)胞、H-727細(xì)胞

NCIH820 Cells;背景說明：乳頭狀肺腺癌；淋巴結(jié)轉(zhuǎn)移；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HBE細(xì)胞、SNU-668細(xì)胞、SKBr3細(xì)胞

NCIH929 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：保持細(xì)胞密度在５×１０５—１×１０６ cells/ml之間，每周換液２—３次;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細(xì)胞樣;相關(guān)產(chǎn)品有：U-251MG細(xì)胞、HuCCT-1細(xì)胞、MARC145細(xì)胞

MC3T3 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：P3-NS1/1-Ag4-1細(xì)胞、Panc 02細(xì)胞、COLO 201細(xì)胞

MeWo Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：５傳代，２-３天換液１次。;生長特性：混合生長;形態(tài)特性：成纖維細(xì)胞;相關(guān)產(chǎn)品有：OCI-Ly3細(xì)胞、HBVSMC細(xì)胞、OPM-2細(xì)胞

SCC-9 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Ls-174-T細(xì)胞、TU 686細(xì)胞、A2780/CP70細(xì)胞

STAN116i-148C4 Cells(提供STR鑒定圖譜)

HLE Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：PLC細(xì)胞、D10G41細(xì)胞、NB4細(xì)胞

WM115mel Cells;背景說明：黑色素瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Panc203細(xì)胞、HTori-3.1細(xì)胞、UCLA RO-81A-1細(xì)胞

BBE Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：６—１：１０傳代，每周換液２次;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：Kupffer細(xì)胞、Y1細(xì)胞、HuCCT-1細(xì)胞

CCC-HEH-2 Cells;背景說明：心肌;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SK-GT-4細(xì)胞、Tn5 B1-4細(xì)胞、MESSADX5細(xì)胞

QSG-7701 Cells;背景說明：該細(xì)胞系來自３５歲女性的肝癌癌旁組織。;傳代方法：消化３-５分鐘，１：２,３天內(nèi)可長滿;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：SW 626細(xì)胞、Y3-Ag1.2.3細(xì)胞、RPTC細(xì)胞

C666-1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：AM細(xì)胞、WI-38細(xì)胞、AU-Mel細(xì)胞

MIA PaCa-2人胰腺癌細(xì)胞代次低|培養(yǎng)基|送STR圖譜

L-540 Cells;背景說明：霍奇金淋巴瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：FRO 81-2細(xì)胞、NCI-H2405細(xì)胞、RL-95-2細(xì)胞

MDA-MB-231-GFP Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：EJ細(xì)胞、LuCL4細(xì)胞、BTI-Tn-5B1-4細(xì)胞

SLMT1 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：L 428細(xì)胞、SK-N-F1細(xì)胞、HEC-1-B細(xì)胞

WB F344 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：T-HSC細(xì)胞、HOP62細(xì)胞、P3-X63-Ag8.653細(xì)胞

NHEK Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MDA 435細(xì)胞、KP-2細(xì)胞、H-1048細(xì)胞

EA hy 926 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞樣;相關(guān)產(chǎn)品有：GM00637B細(xì)胞、NCIH1155細(xì)胞、Sp 2817細(xì)胞

NCIH28 Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：３-１：６傳代，每周換液２-３次;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Human Pancreatic Duct Epithelial細(xì)胞、NTera 2/cl.D1細(xì)胞、MX1細(xì)胞

HS-695T Cells;背景說明：詳見相關(guān)文獻(xiàn)介紹;傳代方法：１：２-１：４傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細(xì)胞;相關(guān)產(chǎn)品有：VP 267細(xì)胞、KRC-Y細(xì)胞、FRTL-5細(xì)胞

BayGenomics ES cell line RRF397 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XG611 Cells(提供STR鑒定圖譜)

EJ-6-2-Bam-6a Cells(提供STR鑒定圖譜)

mSG-PAC1 Cells(提供STR鑒定圖譜)

TRP 7.4 Cells(提供STR鑒定圖譜)

Ma-31 Cells(提供STR鑒定圖譜)

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Nature 417:949-954(2002)

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Thesis VMD (2009); Justus-Liebig-Universitat Giessen; Giessen; Germany

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Non insulin producing cell line, MIA PaCa-2 is rendered insulin producing in vitro via mesenchymal epithelial transition.

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Resolution of novel pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling.

Mol. Cell. Proteomics 15:2671-2685(2016)

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A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076

Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

PubMed=29444439; DOI=10.1016/j.celrep.2018.01.051; PMCID=PMC6343826

Yuan T.L., Amzallag A., Bagni R., Yi M., Afghani S., Burgan W., Fer N., Strathern L.A., Powell K., Smith B., Waters A.M., Drubin D.A., Thomson T., Liao R., Greninger P., Stein G.T., Murchie E., Cortez E., Egan R.K., Procter L., Bess M., Cheng K.T., Lee C.-S., Lee L.C., Fellmann C., Stephens R., Luo J., Lowe S.W., Benes C.H., McCormick F.

Differential effector engagement by oncogenic KRAS.

Cell Rep. 22:1889-1902(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9

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