Human TRAC Protein, His Tag

分子別名（Synonym）

TRAC

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human TRAC, His Tag (TRC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 1 - Ser 115 (Accession # P01848-1).

Predicted N-terminus: Ile 1

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 14.8 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

跨膜蛋白TCR由兩條二硫鍵連接的多肽鏈組成：α和β鏈，γ和δ鏈。每條多肽鏈由一個可變區(qū)和一個恒定區(qū)組成。TRAC是T細(xì)胞受體（TCR）α鏈的恒定區(qū)。TRAC存在于T細(xì)胞表面，并由抗原呈遞細(xì)胞（APC）顯示識別肽主要組織相容性（MH）（pMH）。TRAC參與適應(yīng)性免疫反應(yīng)，在T細(xì)胞治療中得到了很好的研究。