Human Contactin-2 / Tag1 / CNTN2 Protein, His Tag

分子別名（Synonym）

Contactin-2，Tag1，CNTN2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Contactin-2, His Tag (CN2-H5226) is expressed from human 293 cells (HEK293). It contains AA Ser 31 - Asn 1012 (Accession # NP_005067.1).

Predicted N-terminus: Ser 31

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 108.3 kDa. The protein migrates as 116-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

接觸蛋白2也稱為CNTN2、TAX1（瞬時(shí)表達(dá)的軸突糖蛋白）、TAG1（瞬時(shí)軸突糖蛋白）和軸突蛋白1，是免疫球蛋白超家族的成員。CNTN2由六個(gè)Ig樣結(jié)構(gòu)域和四個(gè)纖維連接蛋白III型結(jié)構(gòu)域組成，通過糖基磷脂酰肌醇（GPI）錨定在膜上，而可溶性形式可以通過GPI特異性磷脂酶釋放。作為一種神經(jīng)細(xì)胞粘附分子，由發(fā)育中的中樞神經(jīng)系統(tǒng)和PNS中的一部分神經(jīng)元群體表達(dá)，CNTN2通過與各種粘附分子（包括NgCAM、NrCAM、NCAM和神經(jīng)康）的親同或異親接觸介導(dǎo)細(xì)胞間相互作用。它是一種糖基磷脂酰肌醇（GPI）錨定的神經(jīng)元膜蛋白，起著細(xì)胞粘附分子的作用。它可能在發(fā)育中的神經(jīng)系統(tǒng)中軸突連接的形成中發(fā)揮作用。它也可能參與神經(jīng)膠質(zhì)腫瘤的發(fā)生，并可能為治療干預(yù)提供潛在的靶點(diǎn)。