CD109產(chǎn)品信息

英文名稱：DNA polymerase alpha subunit

中文名稱：DNA聚合酶α亞基

靶點(diǎn)別稱：CD109,CPAMD7

物種：Human

標(biāo)簽：His Tag & Avi Tag

屬性：Protein

標(biāo)記：Biotin-labeled

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human CD109, His,Avitag (CD9-H82E4) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ser 1269 (Accession # Q6YHK3-1).

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 143.5 kDa. The protein migrates as 150-170 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD109是一種糖基磷脂酰肌醇（GPI）連接的糖蛋白，定位于血小板、活化T細(xì)胞和內(nèi)皮細(xì)胞的表面。CD109在許多惡性腫瘤中表達(dá)，包括各種鱗狀細(xì)胞癌和腺癌，并作為多功能輔助受體發(fā)揮作用。據(jù)報(bào)道，CD109與轉(zhuǎn)化生長因子（TGF）-β受體相關(guān)，并負(fù)調(diào)控角質(zhì)形成細(xì)胞中的TGF-β信號(hào)傳導(dǎo)。此外，CD109可能與信號(hào)轉(zhuǎn)導(dǎo)子和轉(zhuǎn)錄激活子-3信號(hào)傳導(dǎo)和異常細(xì)胞增殖有關(guān)。