Human Nogo Receptor / NgR Protein, His Tag (SPR verified)

分子別名（Synonym）

Reticulon-4 receptor，Nogo-66 receptor

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Nogo Receptor, His Tag (NOR-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 27 - Ser 447 (Accession # Q9BZR6).

Predicted N-terminus: Cys 27

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.2 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Nogo受體/網(wǎng)織紅蛋白-4受體在調(diào)節(jié)成人中樞神經(jīng)系統(tǒng)的軸突再生和神經(jīng)元可塑性中起著重要作用。在出生后的大腦發(fā)育中發(fā)揮作用。軸突在大腦中線的正常遷移和胼胝體的正常形成所必需的。保護(hù)運(yùn)動神經(jīng)元免于凋亡；抗凋亡的保護(hù)作用可能是通過與MAG的相互作用介導(dǎo)的。在皮質(zhì)發(fā)育過程中，與RTN4和LINGO1協(xié)同作用，調(diào)節(jié)神經(jīng)元前體細(xì)胞的運(yùn)動。與其他家庭成員一樣，它在限制大腦發(fā)育過程中形成的樹突棘數(shù)量和突觸數(shù)量方面發(fā)揮著作用。