Human GMF-beta Protein, His Tag (MALS verified)

分子別名（Synonym）

GMF-beta,Glia maturation factor beta,GMFB

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human GMF-beta, His Tag (GMA-H5145) is expressed from E. coli cells. It contains AA Ser 2 - His 142 (Accession # P60983).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>95% as determined by SDS-PAGE.

>98% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

膠質(zhì)成熟因子β（GMF-β）是一種17 kDa的蛋白質(zhì)神經(jīng)生長因子，被鑒定為脊椎動(dòng)物大腦中的生長和分化因子。

膠質(zhì)成熟因子β刺激正常神經(jīng)元和膠質(zhì)細(xì)胞的分化。GMFB抑制N-18神經(jīng)母細(xì)胞瘤系和C6膠質(zhì)瘤系的增殖，同時(shí)促進(jìn)其表型表達(dá)。

當(dāng)加入細(xì)胞培養(yǎng)物中時(shí)，GMFβ會(huì)抑制膠質(zhì)細(xì)胞和神經(jīng)元的表型表達(dá)，從而抑制其各自腫瘤的增殖。盡管星形膠質(zhì)細(xì)胞產(chǎn)生GMF-b并將其儲(chǔ)存在細(xì)胞內(nèi)，但它們不會(huì)將GMF-b分泌到培養(yǎng)基中。當(dāng)細(xì)胞直接接觸時(shí)，細(xì)胞表面GMFb在近距離內(nèi)作用于靶細(xì)胞。GMF-β由胸腺上皮細(xì)胞產(chǎn)生，在T細(xì)胞發(fā)育中起著重要作用，有利于CD4+T細(xì)胞。

GMF-β是一種腦特異性蛋白，屬于肌動(dòng)蛋白結(jié)合蛋白（ADF）家族。GMF-β似乎在神經(jīng)系統(tǒng)的分化、維持和再生中發(fā)揮作用。它還支持某些自身免疫性疾病的進(jìn)展，可能是通過其誘導(dǎo)各種促炎細(xì)胞因子的產(chǎn)生和分泌的能力。