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簡單介紹： Fluorescent dyes absorb light at certain wavelengths and in turn emit their fluorescence energy at a higher wavelength. Each dye has a distinct emission spectrum, which can be exploited for multicolor analysis. eBioscience antibodies are available conjugated to a wide variety of fluorochromes.
The tables below lists the properties of the most commonly used fluorochromes in flow cytometry. The eBioscience product number is the prefix used in our catalog numbering system to indicate the corresponding formats of our antibodies. We are continually developing new fluorochrome conjugated antibodies for both flow cytometry and microscopy. Please feel free to contact us at tech@ebioscience.com for more information!
產品編號
熒光染料
激發(fā)波長(nm)
發(fā)射波長 (nm)
熒光強度
使用說明
1=最低熒光強度
5=最高熒光強度
11
FITC (Fluorescein isothiocyanate)
488
518
3
Detected in FL1 channel on most instruments; also a good choice for fluorescence microscopy
53
Alexa Fluor® 488
519
Detected in FL1 channel on most instruments; photostable; an excellent choice for fluorescence microscopy
12
R-PE
575
5
Detected in FL2 channel on most instruments; very high quantum yield (ideal for detection of low density antigens); subject to photobleaching.
(R-Phycoerythrin)
-
PE-Texas Red
615
Detected in FL3 on single laser instruments
PE-Alexa Fluor®610
628
This alternative to PE-Texas Red has less overlap with PE due to its 628nm emission wavelength and is less subject to Fc mediated binding than PE-Cy5.
15
PE-Cy5
670
4
Detected in FL3 or FL4 on most instruments; tandem dye, resonance energy transfer from PE molecule to the cyanine dye Cy5; when used with APC on dual laser machines, needs a cytometer capable of interlaser compensation. See PE-Cy5.5 for dual laser instruments
35
PE-Cy5.5
690
Detected in FL3 or FL4 on most instruments; tandem dye, resonance energy transfer from PE molecule to cyanine dye Cy5.5; when used with APC on dual laser machines, needs a cytometer capable of interlaser compensation. Better choice for dual laser instruments than PE-Cy5.
45
PerCP-Cy5.5
Detected in FL3 or FL4 on most instruments; tandem dye, resonance energy transfer from PerCP molecule to cyanine dye Cy5.5; when used with APC on dual laser machines, needs a cytometer capable of inter-laser compensation. Better choice for dual laser instruments than PE-tandems as a result of its exceptionally large stokes shift.
25
PE-Cy7
760
Detected in appropriate channel based on filter set arrangement of the instrument; tandem dye, resonance energy transfer from PE molecule to cyanine dye Cy7; minimal cross-beam compensation required when used simultaneously
7-AAD
647
Detected in FL3 channel on most instruments; binds selectively to GC regions of DNA; used as a viability dye to discriminate between viable and non-viable cells
Propidium Iodide (PI)
617
Detected in FL2 and FL3 channels on most instruments; a DNA intercalating dye; used as a viability dye to discriminate between viable and nonviable cells
Table 1: Fluorescent Dyes for Flow Cytometric Analysis - 488nm Excitation
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