化合物 3-TYP|T4108|TargetMol

價(jià)格	￥218	￥481	￥823
包裝	1mg	5mg	10mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-12

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產(chǎn)品詳情

中文名稱:化合物 3-TYP	英文名稱:3-TYP
CAS:120241-79-4	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 100.00%	產(chǎn)品類別: 抑制劑
貨號(hào): T4108

Product Introduction

Bioactivity

名稱	3-TYP
描述	3-TYP (3-(1H-1,2,3-triazol-4-yl) pyridine) is a selective SIRT3 inhibitor.
細(xì)胞實(shí)驗(yàn)	Cell viability is analyzed using Cell Counting Kit-8. 1×104 cells are inoculated into 96-well plates. After being treated, 90 μL of medium and 10 μL of CCK-8 solution are added to each well. The cells are then incubated at 37°C for 2 h. After incubation, the absorption at 450 nm is measured using an Infinite? M200 Microplate Reader. The results are expressed as a percentage of the control. The cell death is also evaluated using the trypan blue assay. HepG2 cells are plated in the 6-well plates (5×105 cells per well) and incubated for 24 h. After being treated with Cd or melatonin, the cells are detached with 300 μL trypsin-EDTA solution. The mixture of detached cells is centrifugated at 300 g for 5 min. Then, the residue is combined with 800 μL trypan blue solution and dispersed. After 3 min staining, cells are counted using an automated cell counter. The dead cells are stained with the blue color. Cell mortality (%) is expressed as percentage of the dead cell number/the total cell number.
動(dòng)物實(shí)驗(yàn)	3-TYP is formulated in 1% ethanol.Male C57BL/6 mice are anesthetized with 2% isoflurane, and myocardial ischemia is produced by temporarily exteriorizing the heart via a left thoracic incision and placing a 6-0 silk suture slipknot around the left anterior descending coronary artery. After 30 minutes of myocardial ischemia, the slipknot is released, and the myocardium is reperfused for 3 hour (for western blot analysis and oxidative stress measurement) or 24 hour (for cardiac function, apoptotic index and infarct size determination). Sham-operated mice undergo the same surgical procedures except the suture placed under the left coronary artery is not tied. Ten minutes before reperfusion, mice are randomized to receive either vehicle (1% ethanol) or melatonin (20 mg/kg) by intraperitoneal injection. C57BL/6 mice are randomly divided into the following groups: (i) Sham group: mice underwent the sham operation and are treated with vehicle (1% ethanol); (ii) Mel group: mice are treated with melatonin (20 mg/kg via intraperitoneal injection); (iii) IR+V group: mice underwent the MI/R operation and are treated with vehicle (1% ethanol); (iv) IR+Mel group: mice underwent the MI/R operation and are treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); (v) IR+Mel+3-TYP group: mice are pretreated with 3-TYP (3-TYP is intraperitoneally injected at a dose of 50 mg/kg every 2 days for a total of three doses prior to the MI/R surgery), subjected to the MI/R operation, and treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); and (vi) IR+3-TYP group: mice are pretreated with 3-TYP and then subjected to the MI/R operation.
體外活性	3-TYP顯著減弱了在Cd暴露的HepG2細(xì)胞中，褪黑激素誘導(dǎo)的去乙?；疭OD2表達(dá)和SOD2活性的增加。3-TYP抑制了褪黑激素增強(qiáng)的SIRT3活性，但并不影響SIRT3蛋白表達(dá)。3-TYP預(yù)處理逆轉(zhuǎn)了褪黑激素對(duì)鎘(Cd)引起的線粒體來(lái)源的O2??產(chǎn)生和自噬細(xì)胞死亡的保護(hù)效果。
體內(nèi)活性	3-TYP顯著降低SIRT3活性，增加了SOD2的乙酰化水平，但不影響SIRT3的表達(dá)。3-TYP通過減少LVEF和LVFS，降低了褪黑激素的心臟保護(hù)效應(yīng)，此效應(yīng)在24小時(shí)再灌注后顯著。另外，與IR+Mel組相比，3-TYP增加了梗塞面積、血清LDH水平和凋亡比率。3-TYP（50 mg/kg, i.p.）與Sham組相比，對(duì)LVEF、LVFS、梗塞面積、血清LDH水平、凋亡及氧化應(yīng)激的影響不明顯。此外，與Sham組相比，3-TYP對(duì)gp91phox、Nrf2、NQO 1、Bax、Bcl-2、Caspase-3及活化Caspase-3的表達(dá)水平影響甚微。
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : 5 mg/mL (34.21 mM) Ethanol : 28 mg/mL (191.6 mM) DMSO : 65 mg/mL (444.75 mM)
關(guān)鍵字	Inhibitor \| 3TYP \| 3 TYP \| inhibit \| 3-TYP \| Sirtuin
相關(guān)產(chǎn)品	Fisetin \| Nicotinamide riboside \| BML-278 \| Nicotinamide \| Resveratrol \| Dihydrocoumarin \| Sirtinol \| JGB1741 \| SRT 2104 \| Nicotinamide riboside chloride \| SIRT6-IN-5 \| MC3482
相關(guān)庫(kù)	抑制劑庫(kù) \| 經(jīng)典已知活性庫(kù) \| 抗癌化合物庫(kù) \| 已知活性化合物庫(kù) \| 線粒體靶向庫(kù) \| 組蛋白修飾化合物庫(kù) \| 染色質(zhì)修飾分子庫(kù) \| 抗衰老化合物庫(kù) \| NO PAINS 化合物庫(kù) \| 表型篩選靶點(diǎn)鑒定庫(kù)

關(guān)鍵字： 3-(1H-1,2,3-triazol-4-yl) pyridine|TargetMol

公司簡(jiǎn)介

TargetMol Chemicals Inc. 總部位于馬薩諸塞州波士頓，致力于為全球生化領(lǐng)域科學(xué)家的研究提供專業(yè)的產(chǎn)品和服務(wù)。TargetMol?品牌的客戶群分布于40多個(gè)國(guó)家和地區(qū)，已發(fā)展成為全球知名的化合物庫(kù)和小分子化合物研究供應(yīng)商。 TargetMol?可提供160多種滿足不同需求的化合物庫(kù)，以及多種類型的生化試劑產(chǎn)品，包括12000多種抑制劑、16000多種天然產(chǎn)物和各類多肽、抗體、生命科學(xué)試劑盒等，此外，我們還建設(shè)有CADD（計(jì)算機(jī)輔助藥物設(shè)計(jì)）研究中心、藥理實(shí)驗(yàn)室、藥化合成平臺(tái)三大技術(shù)中心，全方位滿足客戶的定制需求。憑借我們優(yōu)質(zhì)的產(chǎn)品和服務(wù)、快速高效的全球供應(yīng)鏈和專業(yè)的技術(shù)支持，我們將有效幫助您縮短研發(fā)周期，取得更成功的結(jié)果。

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