Human IL-13 R alpha 2 Protein, His Tag (MALS verified)

分子別名（Synonym）

IL13RA2,CD213A2,CT19,IL-13R,IL13BP

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-13 R alpha 2, His Tag (IL2-H52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1).

Predicted N-terminus: Asp 27

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 39.0 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素-13受體亞基α-2也稱(chēng)為IL13Rα2、IL13Ra2分化簇213A2、CD213A2、CT19、IL-13R、IL13BP，是一種膜結(jié)合蛋白，在人類(lèi)中由IL13Ra2基因編碼。IL13Rα2與白細(xì)胞介素-13受體復(fù)合物的亞基IL13Rα1密切相關(guān)。該蛋白以高親和力結(jié)合IL13，但缺乏任何重要的細(xì)胞質(zhì)結(jié)構(gòu)域，似乎不起信號(hào)介體的作用。然而，它能夠調(diào)節(jié)IL13和IL4的作用，盡管它不能直接與后者結(jié)合。據(jù)報(bào)道，它在IL13的內(nèi)化中也發(fā)揮了作用。IL13Rα2是細(xì)胞表面受體的一個(gè)組成部分，然而，大多數(shù)存在于細(xì)胞內(nèi)池中并以可溶性形式存在，因此與IL13Rα1相比，IL13R2作為強(qiáng)效IL13拮抗劑起著相反的作用。它還可能通過(guò)IL13Rα2的短胞內(nèi)結(jié)構(gòu)域和胞質(zhì)結(jié)構(gòu)域與IL4Rα鏈之間的物理相互作用，發(fā)揮IL4依賴(lài)途徑的抑制劑作用。盡管STAT信號(hào)功能失敗，IL13Rα2劑量誘導(dǎo)TGF-β的產(chǎn)生和纖維化。此外，據(jù)報(bào)道，IL13Rα2在多形性膠質(zhì)母細(xì)胞瘤中大量特異性過(guò)表達(dá)。