Human CEACAM-1 / CD66a Protein, His Tag

分子別名（Synonym）

CEACAM1,CD66a,BGP,BGP1,BGPI

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CEACAM-1, His Tag (CE1-H5220) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Gly 428 (Accession # AAH14473).

Predicted N-terminus: Gln 35

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 44.1 kDa. The protein migrates as 57-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>92% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

癌胚抗原相關(guān)細(xì)胞粘附分子1（CEACAM1）也稱為膽汁糖蛋白1（BGP1），CD66a，屬于免疫球蛋白超家族或CEA家族。CEACAM1/CD66a含有三個(gè)Ig樣C2型（免疫球蛋白樣）結(jié)構(gòu)域和一個(gè)Ig樣V型（免疫蛋白樣）的結(jié)構(gòu)域。CEACAM1/CD66a被描述為一種通過親同和異親方式介導(dǎo)細(xì)胞粘附的粘附分子，并在白細(xì)胞、上皮細(xì)胞和內(nèi)皮細(xì)胞上檢測到。研究表明，CEACAM1/BGP-1在多種細(xì)胞過程中發(fā)揮作用，包括組織分化、血管生成、凋亡、轉(zhuǎn)移，以及調(diào)節(jié)先天性和適應(yīng)性免疫反應(yīng)。