Mouse NKG2A / CD159a Protein, His Tag

分子別名（Synonym）

NKG2A,CD159a,KLRC1,NK cell receptor A

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse NKG2A, His Tag (NKA-M52H5) is expressed from human 293 cells (HEK293). It contains AA Thr 95 - Ile 244 (Accession # Q9Z202-1).

Predicted N-terminus: Thr 95

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.1kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

NKG2A/CD159a是一種跨膜蛋白，屬于CD94/NKG2家族的C型凝集素樣受體，可抑制先天免疫系統(tǒng)激活，也稱(chēng)為KLRC1、CD159a、NK細(xì)胞受體a和NKG2-a/NKG2-B II型整合膜蛋白。NKG2A標(biāo)記了一種獨(dú)特的免疫效應(yīng)子亞群，優(yōu)先共表達(dá)組織駐留CD103分子，但不表達(dá)免疫檢查點(diǎn)抑制劑。NKG2A阻斷療法通過(guò)CD8 T細(xì)胞而非NK細(xì)胞進(jìn)行。NKG2A表達(dá)的增加可能是由IL-10誘導(dǎo)的，IL-10在HCC患者的血漿中高水平存在。阻斷IL-10可以特異性抑制NK細(xì)胞中NKG2A的表達(dá)。這些發(fā)現(xiàn)表明，NKG2A的表達(dá)受癌癥巢因子的影響，并導(dǎo)致NK細(xì)胞耗竭，這表明NKG2A阻斷有可能通過(guò)逆轉(zhuǎn)NK細(xì)胞耗竭來(lái)恢復(fù)對(duì)肝腫瘤的免疫力。