Mouse Cathepsin L / CTSL1 Protein, His Tag (MALS verified)

分子別名（Synonym）

CTSL1,MEP,CATL,CTSL

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse Cathepsin L Protein, His Tag (CAL-M52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 18 - Asn 334 (Accession # P06797-1).

Predicted N-terminus: Thr 18

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein contains an Activation peptide, and will be partially processed into Pro form with calculated MW of 35.8 kDa and proteolytic cleavage generate the single-chain activate form with calculated MW of 24.1 kDa and cleavaged activation peptide with calculated MW of 5 kDa under reducing (R) condition. The protein migrates as 40 kDa, 30 kDa and 10 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Supplied as 0.2 μm filtered solution in 25 mM Tris, 150 mM NaCl, pH7.0 with glycerol as protectant.

Contact us for customized product form or formulation.

運(yùn)輸（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

組織蛋白酶L（CTSL1）也被稱為主要排泄蛋白（MEP），是肽酶C1家族的成員，是由二硫鍵連接的重鏈和輕鏈組成的二聚體。CTSL1是一種溶酶體半胱氨酸蛋白酶，在細(xì)胞內(nèi)蛋白質(zhì)分解代謝中起著重要作用。其底物包括膠原蛋白和彈性蛋白，以及α-1蛋白酶抑制劑，這是中性粒細(xì)胞彈性蛋白酶活性的主要控制因素。MEP與多種病理過程有關(guān)，包括肌病和心肌缺血中的肌原纖維壞死，以及對(duì)蛋白尿的腎小管反應(yīng)。CTSL1對(duì)溶酶體中蛋白質(zhì)的整體降解很重要。MEP的特異性接近木瓜蛋白酶。與組織蛋白酶B相比，組織蛋白酶L對(duì)蛋白質(zhì)底物表現(xiàn)出更高的活性，但對(duì)Z-Arg-Arg-NHMec幾乎沒有活性，也沒有肽基二肽酶活性。在pH 4.5至6.5的弱酸性條件下，人組織蛋白酶L活性最大。酶的穩(wěn)定性在較高的pH值下降低。