Mouse MMP-9 (20-471) Protein, His Tag (active enzyme, MALS verified)

分子別名（Synonym）

MMP9,CLG4B,GELB,MANDP2,Gelatinase B

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse MMP-9 (20-471), His Tag (MM9-M52H1) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 471 (Accession # P41245-1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

Predicted N-terminus: Ala 20

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 52.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Supplied as 0.2 μm filtered solution in 25 mM Tris,150 mM NaCl,pH7.5,50% Glycerol with trehalose as protectant.

Contact us for customized product form or formulation.

運(yùn)輸（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

基質(zhì)金屬肽酶9（MMP-9）也稱為92 kDa IV型膠原酶、92 kDa明膠酶或明膠酶B（GELB）、CLG4B，由中性粒細(xì)胞、巨噬細(xì)胞和許多轉(zhuǎn)化細(xì)胞分泌，是結(jié)構(gòu)域結(jié)構(gòu)和活性調(diào)節(jié)方面最復(fù)雜的家族成員。從結(jié)構(gòu)上講，MMP9可分為五個不同的結(jié)構(gòu)域：一個在激活時被切割的前結(jié)構(gòu)域，一個由三個連續(xù)的纖維連接蛋白II型單元組成的凝膠結(jié)合結(jié)構(gòu)域，含有鋅結(jié)合位點(diǎn)的催化結(jié)構(gòu)域，富含脯氨酸的連接區(qū)，以及羧基末端的血紅素樣結(jié)構(gòu)域。這種酶降解各種底物，包括明膠、IV型和V型膠原蛋白以及彈性蛋白。MMP9參與多種自身免疫性疾病，如系統(tǒng)性紅斑狼瘡、類風(fēng)濕性關(guān)節(jié)炎和多發(fā)性硬化癥，被視為潛在的治療靶點(diǎn)。