Human RANK / TNFRSF11A Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

分子別名（Synonym）

CD265,FEO,LOH18CR1,ODFR,OFE,OPTB7,OSTS,PDB2,RANK,TNFRSF11A,TRANCER,CD265 antigen,ODFROSTS,RANKLOH18CR1,NFKB activator

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human RANK Protein, Mouse IgG2a Fc Tag (RAK-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Pro 212 (Accession # Q9Y6Q6-1).

Predicted N-terminus: Ile 30

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 47.2 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

RANK/TNFRSF11A是腫瘤壞死因子受體家族的成員。RANK/TNFRSF11A在其細(xì)胞外四個(gè)富含半胱氨酸的重復(fù)序列中與TNFR家族的其他成員具有顯著的氨基酸同源性。小鼠的功能基因組學(xué)表明，RANKL（TNFSF11）是TNF配體超家族的成員，RANK（TNFRSF11a）是RANKL的同源TNFR家族受體，對(duì)體內(nèi)破骨細(xì)胞生成至關(guān)重要。由成骨細(xì)胞譜系的骨基質(zhì)細(xì)胞表達(dá)的RANKL和由造血髓系譜系的破骨細(xì)胞前體表達(dá)的RANK之間的功能相互作用是破骨細(xì)胞分化、存活和激活所必需的。