Mouse IL-21 Protein, His Tag

分子別名（Synonym）

IL21,Za11,Interleukin-21

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IL-21 Protein, His Tag (IL1-M52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Ser 146 (Accession # Q9ES17).

Predicted N-terminus: Pro 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.3 kDa. The protein migrates as 20 kDa and 23-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素-21（IL-21）是一種分泌蛋白，屬于IL-15/IL-21家族。白細(xì)胞介素-21/IL-21屬于細(xì)胞因子家族，與由私人受體（IL21R）和常見細(xì)胞因子受體γ鏈（γ（C））組成的復(fù)合受體結(jié)合。白細(xì)胞介素-21/IL-21影響多種細(xì)胞類型，包括CD8+記憶T細(xì)胞、NK細(xì)胞和CD4記憶T細(xì)胞亞群。IL-21R廣泛分布于淋巴造血細(xì)胞上。IL-21是CD4+T細(xì)胞在抗原刺激下產(chǎn)生的多效性細(xì)胞因子。它的作用通常會增強免疫細(xì)胞的抗原特異性反應(yīng)。IL-21促進(jìn)CD8+T細(xì)胞和NK細(xì)胞的抗腫瘤活性。IL-21通過與特定的I型細(xì)胞因子受體IL-21R結(jié)合發(fā)揮其作用，IL-21R還含有在其他細(xì)胞因子受體中發(fā)現(xiàn)的γ鏈（γc），包括IL-2、IL-4、IL-7、IL-9和IL-15。IL-21/IL-21R相互作用觸發(fā)一系列事件，包括酪氨酸激酶JAK1和JAK3的激活，隨后是轉(zhuǎn)錄因子STAT1和STAT3的激活。