Mouse IGF-I R / CD221 Protein, His Tag

分子別名（Synonym）

IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IGF-I R, His Tag (IGR-M5223) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1).

Predicted N-terminus: Glu 31 & Asp 742

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.7 kDa and 24.3 kDa respectively. The protein migrates as 45-55 kDa (partial β chain), 100-125 kDa (α chain) and 150 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

胰島素樣生長(zhǎng)因子1受體（IGF1）也被稱為CD221、JTK13。并且是由IGF-1和相關(guān)生長(zhǎng)因子IGF-2激活的跨膜受體。它屬于酪氨酸激酶受體的大類。這種受體介導(dǎo)IGF-1的作用，IGF-1是一種分子結(jié)構(gòu)與胰島素相似的多肽蛋白激素。IGF1R由兩個(gè)α亞基和兩個(gè)β亞基組成，α和β亞基都是由單個(gè)mRNA前體合成的。然后，前體被糖基化、蛋白水解切割，并通過(guò)半胱氨酸鍵交聯(lián)，形成功能性的跨膜αβ鏈。α鏈位于細(xì)胞外，而β亞基橫跨膜，負(fù)責(zé)配體刺激下的細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)。IGF1R具有ATP的結(jié)合位點(diǎn)，ATP用于為自磷酸化提供磷酸鹽。IGF1R和胰島素受體之間有60%的同源性。作為配體結(jié)合的反應(yīng)，α鏈誘導(dǎo)β鏈的酪氨酸自磷酸化。這一事件觸發(fā)了一系列細(xì)胞內(nèi)信號(hào)傳導(dǎo)，雖然有點(diǎn)細(xì)胞類型特異性，但通常會(huì)促進(jìn)細(xì)胞存活和細(xì)胞增殖。