Mouse PSMA / FOLH1 Protein, His Tag (active enzyme) (MALS verified)

分子別名（Synonym）

FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse PSMA, His Tag (PSA-M5245) is expressed from human 293 cells (HEK293). It contains AA Ile 44 - Ala 752 (Accession # O35409-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 81.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in MES and NaCl with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

前列腺特異性膜抗原（PSMA）也被稱為葉酸水解酶1（FOLH1）、谷氨酸羧肽酶2（GCP2）、N-乙酰化α連接酸性二肽酶I（NAALAD1），屬于肽酶M28家族和M28B亞家族。FOLH1/PSMA在pH值大于6.5時(shí)是穩(wěn)定的。FOLH1/PSMA是一種II型跨膜鋅金屬肽酶，在神經(jīng)系統(tǒng)、前列腺、腎臟和小腸中表達(dá)最高。FOLH1/GCP-2是同二聚體，每個(gè)亞基結(jié)合2個(gè)鋅離子，是NAA酶活性所必需的。PSMA的催化活性涉及釋放未取代的C末端谷氨酰殘基，通常來自Ac-Asp-Glu或酰基聚γ-谷氨酸酯。FOLH1/GCP-2/PSMA具有葉酸水解酶和N-乙?；?/span>-α-連接-酸性二肽酶（NAALA酶）活性，并偏愛三α谷氨酸肽。GCP-2/PSMA參與前列腺腫瘤進(jìn)展，并表現(xiàn)出二肽基肽酶IV型活性。在體外，切割Gly-Pro-AMC。