Mouse TSLP Protein, His Tag (MALS verified)

分子別名（Synonym）

TSLP

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse TSLP, His Tag (TSP-M52H8) is expressed from human 293 cells (HEK293). It contains AA Tyr 20 - Glu 140 (Accession # Q9JIE6-1).

Predicted N-terminus: Tyr 20

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

胸腺基質(zhì)淋巴生成素（TSLP）是一種上皮細(xì)胞衍生的細(xì)胞因子，參與炎癥性皮膚病的病理過程，并在上皮細(xì)胞中廣泛表達(dá)。人TSLP cDNA編碼一個159個氨基酸（aa）殘基的前體蛋白，其信號序列為28aa（4,5）。人類TSLP已被證明可以發(fā)展非破壞性中樞耐受，放大上皮誘導(dǎo)的類別轉(zhuǎn)換，誘導(dǎo)特應(yīng)性疾病，并維持腸道非炎癥環(huán)境。在對人TSLP有反應(yīng)的多種細(xì)胞中，CD11c+樹突狀細(xì)胞是特征最明顯的靶細(xì)胞。