| 名稱 | Oxymatrine |
| 描述 | Oxymatrine (Oxysophoridine) is an alkaloid isolated from Sophora flavescens, used as the antibiotic. It is a traditional Chinese medicine used in the treatment against hepatitis B virus.It can also inhibit iNOS expression and TGF - β / Smad pathway. |
| 細(xì)胞實(shí)驗(yàn) | DU145, PC-3 and PNT1B cell lines (3×104 cells/well) are seeded into 96-well plates and incubated overnight at 37°C in 5% CO2. Subsequently, the cells are incubated with different concentrations of oxymatrine (0, 2, 4, 6 and 8 mg/mL). MTT (10 mL; 5 mg/mL) is added and the mixture is incubated in darkness at 37°C for 2 h. Absorbance is measured at a wavelength of 490 nm using a microplate reader[2]. |
| 激酶實(shí)驗(yàn) | P450 Inhibition Studies: Pooled human liver microsomes (HLM) from at least 15 donors are used for all inhibition assays. For IC50 determinations, the substrate probes are used at their approximate in vitro Km values. All incubations are performed with 100 mM potassium phosphate buffer (pH 7.4) and 1 mM NADPH. For CYP1A2 inhibition study, incubations are performed in a total volume of 0.5 ml, in duplicates with 0.1 mg/ml HLM, 30 μM phenacetin, 1 mM NADPH, and in the presence of avasimibe (0, 0.3, 0.75, 1.5, 3, 7.5, 15, 30, and 40 μM in 50 mM) in a potassium phosphate buffer at pH 7.4. After preincubation at 37 °C for 7 min, NADPH is added to initiate the enzyme reaction. The reaction mixture is quenched with 500 μl of ice-cold 100 ng/ml paracetamol-D4/CH3CN after 25 min. The standards (4-acetamidophenol, singlet) and quality controls (triplicates for low, medium, and high) are prepared at room temperature. After mixing, 0.2 ml of the samples is transferred to another plate and submitted for LC/MS/MS analysis after centrifugation at 3000 rpm for 10 min. A Supelco Discovery Amide C16, 100 × 2.1 mm (5-μm particle size) column (Supelco, Bellefonte, PA) is used. The mobile phase is isocratic, 40:60 [acetonitrile/formic acid, 0.1% (v/v)] at 0.2 ml/min. |
| 體內(nèi)活性 | 在小鼠中的LD50為521 mg/kg，經(jīng)腹腔注射(i.p.)。 |
| 存儲(chǔ)條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 100 mg/mL (378.27 mM), Sonication is recommended.
H2O : 33.33 mg/mL (126.09 mM), Sonication is recommended.
|
| 關(guān)鍵字 | TGF-beta/Smad | Inhibitor | inhibit | Transforming growth factor beta | Influenza Virus | Oxymatrine |
| 相關(guān)產(chǎn)品 | Guanidine hydrochloride | Naringin | Valproic Acid | Taurine | Gefitinib | Aceglutamide | Hydroxychloroquine | Curcumin | Stavudine | Acetylcysteine | Paeonol | Sodium 4-phenylbutyrate |
| 相關(guān)庫(kù) | 經(jīng)典已知活性庫(kù) | 抗癌上市藥物庫(kù) | 抗癌活性化合物庫(kù) | 已知活性化合物庫(kù) | 抗病毒庫(kù) | TGF-β/Smad靶點(diǎn)化合物庫(kù) | 藥物功能重定位化合物庫(kù) | 中藥抗炎分子庫(kù) | 抗感染天然產(chǎn)物庫(kù) |