Human CD206 / MMR Protein, His Tag

分子別名（Synonym）

MMR,CD206,hMR,MRC1,CLEC13D,CLEC13DL,MRC1L1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CD206, His Tag (CD6-H52H9) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Ala 1389 (Accession # P22897-1).

Predicted N-terminus: Leu 19

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 158.6 kDa. The protein migrates as 150-190 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Mrc1是一種保守的檢查點(diǎn)介導(dǎo)蛋白，它將復(fù)制應(yīng)激信號(hào)轉(zhuǎn)導(dǎo)給下游效應(yīng)激酶。Mrc1及其脊椎動(dòng)物同源物Claspin充當(dāng)復(fù)制應(yīng)激檢查點(diǎn)信號(hào)傳導(dǎo)的介質(zhì)，從Mec1/Rad3/ATR傳感器激酶接收信號(hào)并將其傳遞給效應(yīng)器Rad53/Cds1/Chk1激酶。mrc1檢查點(diǎn)活性的喪失導(dǎo)致羥基脲存在時(shí)晚期/休眠起源的異常激活。表達(dá)多配體內(nèi)吞受體甘露糖受體（CD206/MRC1）的腫瘤相關(guān)巨噬細(xì)胞（TAM）有助于腫瘤免疫抑制、血管生成、轉(zhuǎn)移和復(fù)發(fā)。