Human CD200 / OX-2 Protein, His Tag

分子別名（Synonym）

CD200,MOX1,MOX2,MRC,OX-2,My033

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CD200, His Tag (OX2-H5228) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gly 232 (Accession # AAH22522).

Predicted N-terminus: Gln 31

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.3 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>98% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD200也被稱為OX-2膜糖蛋白（OX-2），是一種1型膜糖蛋白，含有兩個免疫球蛋白結(jié)構(gòu)域（1個Ig樣C2型（免疫球蛋白樣）結(jié)構(gòu)域和Ig樣V型（免疫蛋白樣）功能域），因此屬于免疫球蛋白超家族。CD200/OX-2在多種細(xì)胞類型中廣泛表達(dá)。CD200與主要在髓系細(xì)胞上表達(dá)的結(jié)構(gòu)相關(guān)受體（CD200R）相互作用，參與巨噬細(xì)胞和肥大細(xì)胞功能的調(diào)節(jié)。OX-2/CD200和CD200R通過其各自的N-末端Ig樣結(jié)構(gòu)域結(jié)合。CD200在預(yù)防移植物排斥反應(yīng)、自身免疫性疾病和自然流產(chǎn)方面也起著重要作用。