Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His Tag&Tag Free
分子別名（Synonym）
Integrin alpha V beta 1,ITGAV&ITGB1
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)).
Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.9 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 125-150 kDa (ITGAV) and 95-110 kDa (ITGB1) respectively due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>90% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
整合素α-5/β-1是纖維蛋白原的受體。整合素α-1/β-1、α-2/β-1，α-6/β-1和α-7/β-1是lamimin的受體。整合素α-4/β-1是VCAM-1的受體。它識(shí)別VCAM-1中的序列Q-I-D-S。整合素α-9/β-1是VCAM-1、細(xì)胞觸覺(jué)素和骨橋蛋白的受體。它識(shí)別細(xì)胞觸覺(jué)素中的序列A-E-I-D-G-I-E-L。整合素α-V/β-1也是玻連蛋白的受體。Beta-1整合素在多種配體中識(shí)別R-G-D序列。異構(gòu)體2干擾異構(gòu)體1，對(duì)細(xì)胞粘附和遷移（體外）產(chǎn)生顯性負(fù)面影響。當(dāng)與α-7/β-1整合素結(jié)合時(shí)，調(diào)節(jié)細(xì)胞粘附和層粘連蛋白基質(zhì)沉積。
關(guān)鍵字： 整合素αvβ1;Integrin αvβ1;αvβ1;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國(guó)生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國(guó)、美國(guó)、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶(hù)超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長(zhǎng)期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。