Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His Tag&Tag Free
分子別名（Synonym）
Integrin alpha V beta 8,ITGAV&ITGB8
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-H52W4) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)).
Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 112.9 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 80-90 kDa (ITGB8) respectively due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
整合素αVβ8（ITGAV和ITGB8或ITGAVB8）在卵黃囊、胎盤、腦血管周圍星形膠質(zhì)細(xì)胞、雪旺氏細(xì)胞、腎小球系膜細(xì)胞和肺上皮細(xì)胞中表達(dá)。與其他αV整合素不同，ITGAVB8似乎沒有呈現(xiàn)不同的激活狀態(tài)，細(xì)胞質(zhì)尾部也沒有連接到細(xì)胞骨架。它結(jié)合含有RGD基序的配體，包括玻連蛋白、纖維蛋白和潛在TGF-β復(fù)合物的潛伏相關(guān)肽（LAP）。αVβ8與LAP的高親和力結(jié)合允許MT1-MMP進(jìn)行蛋白水解切割，從而釋放活性TGF-β。這種機(jī)制不同于αVβ6，另一種αV整合素可以通過非蛋白水解機(jī)制從潛伏期激活TGF-β。TGF-β激活的下游效應(yīng)包括控制細(xì)胞生長和相關(guān)的血管化。
關(guān)鍵字： Integrin avb8;整合素avb8;αvβ8;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。